The influence of in vitro aggregation of apheresis platelets on their quality
10.13303/j.cjbt.issn.1004-549x.2025.12.014
- VernacularTitle:单采血小板体外聚集对其质量影响的研究
- Author:
Huizhong LIU
1
;
Huaheng LI
1
;
Dawei CHEN
1
;
Daixiao OU
1
;
Huibin ZHONG
1
;
Yue ZHANG
1
;
Huaqin LIANG
1
Author Information
1. Guangzhou Blood Center, Guangzhou 510095, China
- Publication Type:Journal Article
- Keywords:
in vitro aggregation;
apheresis platelets;
CD62p expression rate;
hemostatic function
- From:
Chinese Journal of Blood Transfusion
2025;38(12):1742-1746
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the impact of disaggregation on the quality of in vitro aggregated apheresis platelets. Methods: The apheresis platelets collected from Guangzhou Blood Center served as the study samples. The in vitro aggregated apheresis platelets after successful disaggregation were designated as the experimental group (referred to as the aggregation group), and apheresis platelets without in vitro aggregation during collection served as the control group. The product volume, platelet content, residual white blood cells, red blood cell contamination, pH value, CD62p expression rate, platelet morphology score and thromboelastography of both groups were respectively detected. Results: The routine quality control indicators such as product volume, platelet content, residual white blood cells, red blood cell contamination, and pH value of both groups all met the quality requirements. There were statistically significant differences in pH value (7.180 vs 7.071) between the two groups (P<0.05). There was no significant difference in product volume, platelet content, residual white blood cells, and red blood cell contamination between the two groups (P>0.05). The CD62p expression rate of the aggregation group was higher than that of the control group (42.386% vs 17.310%, P<0.05), while the cell morphology score of the aggregation group was lower than that of the control group (132.066 vs 141.166, P<0.05). No statistically significant differences were found in thromboelastography parameters (R-CK, K-CK, α angle, MA-CK, CI-CK) between the two groups (P>0.05). Conclusion: After the disaggregation of in vitro-aggregated apheresis platelets, the quality indicators met the national quality requirements. Although the expression rate of CD62p increased and the cell morphology score decreased, there were no statistically significant differences in the thromboelastography parameters between the two groups. This indicates that although some platelet activation occurred, it did not affect the hemostatic function of the platelets.
