Effects of HLA & HPA alleles and haplotypes on establishing platelet donor databases
10.13303/j.cjbt.issn.1004-549x.2025.12.012
- VernacularTitle:血小板捐献者资料库HLA、HPA等位基因及单体型对建库的影响分析
- Author:
Zhifen YANG
1
;
Yi JIANG
1
;
Jie CHEN
1
;
Liqun CAO
1
;
Qing HU
1
;
Tian KANG
1
;
Yubin XIE
1
Author Information
1. Changsha Blood Center, Changsha 410001, China
- Publication Type:Journal Article
- Keywords:
apheresis platelet donor database;
human leukocyte antigen;
human platelet antigen;
high-resolution genotyping;
haplotype frequency
- From:
Chinese Journal of Blood Transfusion
2025;38(12):1728-1734
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the distribution of HPA, HLA alleles and haplotypes among apheresis platelet donors in Changsha, China, and to establish an apheresis platelet donor database. Methods: High-resolution genotyping of HLA-A and -B was performed using PCR sequence based typing (SBT) and next generation sequencing (NGS). HPA genotyping was conducted using quantitative PCR (Q-PCR). The allele frequency, haplotype frequency and linkage disequilibrium parameters were calculated using the direct counting method, the maximum likelihood method and Arlequin software (V 3.1). Results: A total of 41 HLA-A alleles and 82 HLA-B alleles were detected, and 457 HLA-A-B haplotypes were found, of which 25 showed strong linkage disequilibrium (RLD>0.50). The HPA-3 and HPA-15 had the highest HPA polymorphism and antigen mismatch rate in apheresis platelet donor database in Changsha, and the dual antigen mismatch rate was 0.3704 and 0.3743, respectively. Conclusion: The polymorphism of apheresis platelet donor database in Changsha is complex and has strong regional characteristics. Establishing a high-resolution donor database will strongly support the provision of genetically matched platelets for clinical use, facilitating precise platelet transfusion therapy.
