Effect of Angiostrongylus Cantonensis Infection on the Immune System in Different Hosts
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0509
- VernacularTitle:广州管圆线虫感染对不同宿主免疫系统的影响
- Author:
Zhen LIU
1
;
Jia-hua LIU
2
;
Zhi-xuan MA
3
;
Zong-pu ZHOU
3
;
Zhong-dao WU
2
;
Ying FENG
3
Author Information
1. Department of Blood Transfusion， Guangzhou First People’s Hospital // the Second Affiliated Hospital of South China University of Technology，Guangzhou 510180 ，China
2. Department of Parasitology， Zhongshan School of Medicine， Sun Yat-sen University， Guangzhou 510080， China
3. School of Medicine， South China University of Technology， Guangzhou 510006 ， China
- Publication Type:Journal Article
- Keywords:
Angiostrongylus cantonensis;
rat;
mouse;
spleen;
thymus
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(5):714-720
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of host immune system in the pathogenesis of encephalitis induced by Angiostrongylus cantonesis （A.C.） infection. MethodsThe definitive hosts of A.C. are rats and the intermediate hosts are mice. SD rats and BALB/c mice infected with A.C. were used to observe the changes of central immune organ thymus and peripheral immune organ spleen after 21 days of infection. Flow cytometry was used to detect the changes of cell subtypes in the thymus and spleen of rats and mice . ResultsAfter 21 days of infection with A.C. in rats， there was no obvious change in the appearance of spleen and thymus gland， and no obvious change in the thymus cell total count and in the proportion and number of thymocyte subtype cell （CD4+T， CD8+T， CD4+ CD8+T and CD4- CD8-T cells） between control groups and infected groups were found（P>0.05）； The proportion and number of CD4+T cells in spleen was increased（P=0.016；P=0.023）， while the change of CD8+T cells was not obvious（P>0.05）. After 21 days of infection， the spleen and thymus of mice were severely atrophied， the total number of thymus cell was decreased sharply（P<0.000 1）；The proportion and number of CD4+CD8+T cells were decreased obviously（P<0.000 1；P<0.000 1）； Infection resulted in a decrease in the total number of mononuclear cells in the spleen of mice（P<0.000 1）；The number of CD4+T cells and CD8+T cells in spleen was increased（P<0.000 1； P=0.003 8）. ConclusionsA.C. infection severely suppressed the immune system of mice， but had little effect on rats. A.C. infection induced immunosuppression in the host， in turn reduced the ability of the host to clear and resist parasites， which may be an important mechanism of immune escape of parasites. This study revealed that the immune system plays an important role in the pathogenesis of encephalitis caused by A.C. infection， which provides a new idea for the prevention and treatment of encephalitis caused by A.C. infection.
