Expression and Clinical Significance of TLR4 and MyD88 in Esophageal Squamous Cell Carcinoma
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0120
- VernacularTitle:TLR4和MyD88在食管鳞癌组织中的表达及临床意义
- Author:
Xu-yang LIANG
1
;
Ping XU
2
;
Sheng-xiang LV
1
;
Zhi-mei ZHANG
1
;
Lu WANG
1
;
Shu-xian ZHANG
1
;
Ling REN
1
;
Yan-qin MA
1
Author Information
1. Department of Gastroenterology， Lianyungang Clinical College of Nanjing Medical University// The First People's Hospital of Lianyungang， Lianyungang 222061， China
2. Department of Gastroenterology， Shanghai Songjiang Clinical Medical College of Nanjing Medical University， Shanghai 201600， China
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
TLR4;
MyD88;
proliferating cell nucleus antigen;
vascular endothelial growth factor
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(3):475-481
- CountryChina
- Language:Chinese
-
Abstract:
ObjectivesTo investigate the protein expressions of toll-like receptor 4 （TLR4） and myeloid differentiation factor 88 （MyD88） in esophageal squamous cell carcinoma （ESCC） and their relationship to the related pathological factors and the clinical significance. MethodsTotally 72 ESCC specimens and paracancerous normal tissue specimens were selected. EnVision immunohistochemical staining was used to detect the protein expression levels of TLR4， MyD88， proliferating cell nucleus antigen （PCNA） and vascular endothelial growth factor （VEGF） in ESCC and in paracancerous normal tissue， and Multivariate Logistic stepwise regression analysis was used to analyze their expressions and clinicopathological factors. ResultsThe protein expressions of TLR4， MyD88， PCNA and VEGF in ESCC were significantly higher than those in normal tissues （P=0.031，P=0.011，P=0.012，P=0.022）. Multivariate Logistic stepwise regression analysis showed that the independent risk factors of TLR4， MyD88， PCNA and VEGF protein expression levels were TNM stage （P=0.032，P=0.005， P=0.000， P=0.003）， rather than the genders， ages， depth of tumor invasion， or degree of differentiation. There was a positive correlation between the protein expressions of TLR4 and MyD88 in ESCC （r= 0.618， P˂0.01）. The protein expressions of MyD88 and PCNA， MyD88 and VEGF were positively correlated （r= 0.516， P˂0.01； r= 0.708， P˂0.01）. ConclusionsTNM stage is an independent risk factor of the protein expression levels of TLR4， MyD88， PCNA and VEGF， and the expression of MyD88 protein is positively correlated with expression of TLR4， PCNA and VEGF. It indicates that TLR4-MyD88 signaling pathway can promote the occurrence and development of ESCC. The combined detection of TLR4 and MyD88 may be helpful to evaluate the malignant degree of ESCC. Therefore， TLR-MyD88 signaling pathway may be used as an important biological indicator to reflect the prognosis of ESCC and an important target of anti ESCC.
