Analysis on In Vitro Culture and Clinical Outcomes of Embryo Contamination During IVF-ET Cycles
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0114
- VernacularTitle:IVF-ET周期中胚胎污染的实验室与临床结局分析
- Author:
Feng-jiao DU
1
;
Rui-qi LI
1
;
Yu-qin ZHU
1
;
Neng-yong OUYANG
1
;
Song-bang OU
1
;
Wen-jun WANG
1
Author Information
1. Reproductive Medicine Centre， Department of Obstetrics and Gynecology， Sun Yat-sen Memorial Hospital， Sun Yat-sen University， Guangzhou 510120， China
- Publication Type:Journal Article
- Keywords:
in vitro fertilization and embryo transfer （IVF-ET）;
embryo contamination;
semen;
bacteria
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(3):432-440
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the occurrence， in vitro culture effect and clinical outcomes of embryo contamination during in vitro fertilization and embryo transfer （IVF-ET） cycles. MethodsA retrospective analysis was made on the embryo contamination cases during IVF-ET treatment at Reproductive Medicine Centre， Department of Obstetrics and Gynecology， Sun Yat-sen Memorial Hospital， Sun Yat-sen University from January 2010 to December 2020. We compared the contamination of 4 different insemination methods， contamination on 3 different in vitro culture days （D1， D2， D3） and contamination of 3 different samples （follicular fluid， semen and culture medium）. The source of contamination and types of microbes were identified. We also compared in vitro culture and clinical outcomes between two groups （42 cases of all embryos contaminated and 28 cases of partial embryos contaminated）. ResultsAmong the 29 583 cycles examined， 70 cycles had microbial contamination （0.24%）， and all contaminated embryos were from in vitro fertilization （IVF） source rather than intracytoplasmic sperm injection （ICSI） source. The contamination rate on D2 was the highest （54.3%）， followed by those on D1 （32.9%） and D3 （12.9%）. Compared with follicular fluid， semen is the most common source of contamination from D1-D3. The most common contaminants identified was Enterococcus faecalis in follicular fluid （18.0%）， while it was Escherichia coli in semen （59.6%） and culture medium （66.7%）. Moreover， the types of bacteria in culture medium were not consistent with those in follicular fluid and semen in 5 cases of microbial contamination. Compared with the Total contaminated group， the Partial contaminated group showed a significant decrease in No available embryo rates （6/28 vs. 34/42， 21.4% vs. 81.0%， P<0.001） and a rising trend in formation of blastocyst rates （5/12 vs. 2/7， 41.2% vs. 28.6%， P=0.656）， meanwhile， the clinical pregnancy cases and live births cases after transplantation of fresh and frozen-thawed cycles in Partial contaminated group were higher than those in the Total contaminated group. ConclusionICSI can effectively reduce embryo contamination. Embryo contamination mainly occurred on D2 in vitro culture and the most common contamination source is Escherichia coli in semen. Partial embryo contamination may still result in good in vitro culture effect and clinical outcomes during IVF-ET cycles.
