Association of Plasma Exosomal miRNAs with Gestational Diabetes Mellitus
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0108
- VernacularTitle:血浆外泌体miRNAs与妊娠期糖尿病的关系
- Author:
Zhi-xin YE
1
;
Du-bo CHEN
2
;
Pei-song CHEN
2
;
Sui-wen LIN
1
;
Xiao-qing HUANG
1
;
Shi-qi LI
1
;
Bin LIU
1
Author Information
1. Department of Obstetrics and Gynecology， The First Affiliated Hospital， Sun Yat-sen University， Guangzhou 510080， China
2. Department of Clinical Laboratory， The First Affiliated Hospital， Sun Yat-sen University， Guangzhou 510080， China.
- Publication Type:Journal Article
- Keywords:
gestational diabetes mellitus;
microRNA;
exosomes
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(3):381-391
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of diabetes related microRNA （miRNA） in plasma exosomes of pregnant women complicated with gestational diabetes mellitus （GDM） and to study their association with the pathogenesis of GDM. MethodsIn this study， diabetes related miRNAs microarray data sets were searched through Gene Expression Omnibus （GEO） database， and screened by GEO2R to determine the candidate miRNAs. Differentially expressed miRNAs were selected if the change is consistent in more than one microarray data sets. Singleton pregnant women within 10-16 gestational weeks （gws） were included and their plasma was obtained for further analysis. In 24~28 gws， oral glucose tolerance tests （OGTT） were performed to diagnose GDM or normal glucose tolerance （NGT）. Diabetes related miRNAs expression in plasmatic exosomes was compared between GDM and NGT groups by RT-PCR. Receiver operating characteristic （ROC） curve was applied to assess the predictive efficiency of miRNAs. Finally， Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis was used to reveal the function of miRNAs. ResultsFive diabetes related microarray datasets were downloaded from GEO database， including GSE94649， GSE21321， GSE98043， GSE148961 and GSE27645. Ten differentially expressed miRNAs were found， among which seven were up-regulated and three were down-regulated. RT-PCR results showed that， compared with NGT group， hsa-miR-188-5p， hsa-miR-663a， hsa-miR-135a-5p and hsa-miR-4707-5p in plasma exosomes of GDM pregnant women were up-regulated. The area under the ROC curve （AUC） of these four miRNAs for GDM prediction was 0.839 （95%CI：0.724~0.954）. The target genes of miR-135a-5p were involved in insulin signaling pathways. ConclusionsDifferentially expressed exosomal miRNAs in GDM plasma act as potential predictors of GDM. Among them， miR-135a-5p mainly participates in insulin signaling pathways.
