Treatment with Compound Danshen Dripping Pills Improves Cardiac Function in Rats following Ischemia-Reperfusion through Reducing Endothelial to Mesenchymal Transition in Microvessels within Heart Tissue
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0105
- VernacularTitle:复方丹参滴丸减少心肌微血管内皮-间质转化保护缺血-再灌注大鼠的心功能
- Author:
You-gang MA
1
;
Feng-guang KANG
2
;
Ru-lin XU
1
;
Lan XU
3
;
An-ping CAI
3
;
Fan-fang ZENG
4
;
Li-wen LI
3
;
Wei-yi MAI
1
Author Information
1. Department of Cardiology， The First Affiliated Hospital of Sun Yat-sen University // NHC Key Laboratory of Assisted Circulation （Sun Yat-sen University）， Guangzhou 510080， China
2. Department of Cardiology， Shunde Hospital of Guangzhou University of Traditional Chinese Medicine， Foshan 528300， China
3. Department of Cardiology， People's Hospital of Guangdong Province， Guangzhou 510080， China
4. Department of Cardiology， Shenzhen Branch of Fuwai Hospital， Chinese Academy of Medical Sciences， Shenzhen 518057， China
- Publication Type:Journal Article
- Keywords:
compound Danshen dripping pills;
myocardial infarction;
ischemia reperfusion;
endothelial to mesenchymal transition
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(3):355-363
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe present study was conducted to investigate the protective effect on cardiac function and potential mechanism of Compound Danshen Dripping Pills （CDDPs） on myocardial ischemia reperfusion in rats. MethodsThirty two male SD rats were underwent cardiac reperfusion following 45 minutes of left anterior descending coronary artery ligation， and randomly divided into 4 groups （n= 8 ）， rats in each group were given different doses of CDDPs （40，80，120 mg·kg-1·d-1 ）， or normal saline （control group） by gavage. Another 8 rats underwent similar procedure but without LAD ligation were set as sham group （were also given same volume of normal saline by gavage）. The treatment lasted for 4 weeks. Then echocardiography was conducted to evaluate the end-point cardiac function. HE and Masson’s trichrome staining were performed to observe the change of histomorphology and fibrosis. CD31/α-SMA immunofluorescence was implemented to investigate the endothelial to mesenchymal transition in cardiac microvessels. Western Blot was used to analyze the expression of α-SMA and CD31 in ventricular tissue of infarcted border zone. Data were analyzed by one-way ANOVA or Kruskal-Wallis H non-parametric test. ResultsCompared with normal saline group， treatment with different doses of CDDP could increase ejection fraction and fractional shortening significantly （P＜0.05 at least）， decrease left ventricular end-diastolic volume and the E/A ratio significantly （P＜0.05 at least）， reduce the cardiac collagen volume fraction （both P<0.05）， and suppress the expression of mesenchymal marker α-SMA in cardiac microvessels detected by immunofluorescent staining（P＜0.05 at least）， and decrease the expression of α-SMA and increase the expression of CD31 in ventricular tissue of infarcted border zone detected by Western blot. ConclusionTreatment with 40/80/120 mg·kg-1·d-1 doses of CDDPs for 4 weeks could improve cardiac function in rats underwent ischemia-reperfusion， this might be through reducing the occurrence of endothelial to mesenchymal transition in microvessels within heart tissue， and subsequently decreasing the cardiac fibrosis.
