Establishment of Chronic Stress Inducing Depressive Disorder Mouse Model Via Chemical Genetics
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0104
- VernacularTitle:基于化学遗传的慢性应激导致抑郁症小鼠模型的构建
- Author:
Yi-wen DENG
1
;
Yin-hang ZHANG
1
;
Xing-hao XU
1
;
Jun-zhe YI
1
;
Xiao-ran ZHANG
1
;
Wei-jun HUANG
1
Author Information
1. Center for Stem Cell Biology and Tissue Engineering， Zhongshan School of Medicine， Sun Yat-sen University， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
depressive disorder;
chronic stress;
sympathetic nerve;
chemical genetics;
RVLM
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(3):346-354
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a chronic stress inducing depressive disorder mouse model via chemical genetics， that helps to optimize the animal modeling method of chronic stress-induced depressive disorder. MethodsTo continuously stimulate the rostral ventrolateral medulla （RVLM） of mouse by chemical genetic method to mimic the response to chronic stress. The changes about behavior， blood pressure and major inflammatory factors of the animal models were correspondingly observed. ResultsThe mouse model was established successfully. One hour after intraperitoneal administration of CNO， diastolic blood pressure and mean blood pressure were significantly increased in the model group compared with the control. After four weeks of continuous administration， the total distance of movement， the total entering bouts， the distance and duration of the central area in the model group were significantly decreased compared with the control in the open field experiment. The results of sucrose preference and rotarod experiment suggested anhedonia and weakened motor coordination ability in the model group. The heart rate， blood pressure， expressions of IFN-γ and IL-10 in the model group were higher than those in the control. ConclusionsOur model developed classic symptoms of depressive disorder efficiently and stably， as well as accompanying symptoms such as high blood pressure and mussy release of inflammatory factors. It provides a valuable scientific basis for improving the efficiency and stability of animal modeling of chronic stress-induced depressive disorder.
