Fruitflow， a Water-soluble Tomato Extract， Regulates Platelet Oxidative Damage via Autophagy in Vitro
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0101
- VernacularTitle:水溶性番茄浓缩物经自噬途径调控血小板的氧化损伤
- Author:
Kong-yao LI
1
;
Yi-lin SHI
2
;
Xi-lin MA
1
;
Ze-zhong TIAN
1
;
Jin-chao ZOU
1
;
Rui-jie WANG
1
;
Yu-heng MAO
1
;
Yan YANG
1
Author Information
1. School of Public Health，Shenzhen， Sun Yat-sen University， Shenzhen 518106， China
2. Guangdong Provincial Key Laboratory of Food， Nutrition and Health， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
Fruitflow;
platelet;
oxidative damage;
autophagy
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(3):321-327
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveExcessive oxidative stress can promote platelet oxidative damage and plays an important role in the occurrence and development of cardiovascular diseases. Fruitflow， a water-soluble tomato extract， can inhibit platelet aggregation and activation， but it has not been reported whether Fruitflow can reduce platelet oxidative damage through autophagy. Therefore， we conducted this study to investigate the effect of Fruitflow on oxidative damage in H2O2-treated platelets as well as the underlying mechanism in vitro. MethodsGel-filtered platelets from healthy people were pre-incubated with different concentrations （0， 20， 40， 80 mg/L） of Fruitflow for 30 minutes， and then treated with H2O2 （1 mmol/L） for another 60 minutes. The level of mitochondrial membrane potential（ΔΨm）depolarization was determined by flow cytometry. Enzyme-linked immunosorbent assay was used to detect reactive oxygen species （ROS） level. The expression of p53， phospho-p53， LC3Ⅱ/Ⅰ， and p62 was detected by Western blot. ResultsFruitflow significantly inhibited the ΔΨm depolarization， ROS production and p53 phosphorylation in H2O2-treated platelets （P<0.05）. The autophagy inhibiter （3-MA） significantly reversed the effect of Fruitflow on reduction of platelet ROS generation （P<0.05）. In addition， Fruitflow reduced p62 and increased the expression level of LC3Ⅱ/Ⅰ in resting platelets （P<0.05）， and increased the expression level of LC3Ⅱ/Ⅰ in H2O2-treated platelets （P<0.05）. Furthermore， the effect of Fruitflow on decreasing ΔΨm depolarization in H2O2-treated platelet was reversed by 3-MA （P<0.05）. ConclusionFruitflow can significantly reduce the H2O2-induced platelet oxidative damage by promoting autophagy in vitro.
