Preparation and evaluation of folic acid-modified liposomal vaccines based on natural IgM adjuvant
10.12206/j.issn.2097-2024.202401060
- VernacularTitle:基于天然IgM新型佐剂的叶酸脂质体疫苗制备及评价
- Author:
Anze LIU
1
;
Yingying HE
1
;
Huan WANG
1
;
Ying LU
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
vaccines;
liposomes;
nano-vaccine;
IgM functionalization;
folic acid
- From:
Journal of Pharmaceutical Practice and Service
2025;43(11):555-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective To increase the antigen presentation and immune response of liposomal vaccines through IgM functionalization strategy. Methods Folic acid-modified liposomes loaded with ovalbumin （FA-sLip/OVA） were prepared. The binding ability of liposomal vaccines with IgM was investigated by ELISA method. The IgM in the protein corona was analyzed by SDS-PAGE and Western Blot. Mice were immunized by intravenous injection to investigate the spleen B cell targeting and immune activation of vaccines. Results The average particle size of FA-sLip/OVA was about 117 nm. The specific binding between FA-sLip and IgM was verified by ELISA method. Folic acid-modified liposomal vaccines could absorb IgM in mouse serum and form protein coronas on the surface. Intravenous immunization could stimulate mice to produce long-lasting, high-level antibody titers. Conclusion Natural IgM-enabled folic acid-modified liposomal vaccines were successfully prepared which could effectively induce immune response and provide a new idea for the research of nano-vaccines.
