Analysis of the chemical constituents of Maxing Shigan decoction by UPLC-Q-TOF/MS
10.12206/j.issn.2097-2024.202306028
- VernacularTitle:基于超高效液相色谱-四极杆飞行时间质谱的麻杏石甘汤化学成分分析
- Author:
Xue ZHAO
1
;
Yanqiu GU
2
;
Haowen CHU
3
;
Caisheng WU
4
;
Gao LI
1
;
Xiaofei CHEN
5
Author Information
1. School of Pharmacy, Yan Bian University, Yanji 133002, China.
2. Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China.
3. Unit 32268 of the PLA, Dali 671003, China.
4. School of Pharmacy, Xia Men University, Xiamen 361005, China.
5. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
Maxing Shigan decoction;
chemical composition;
COVID-19;
UPLC-Q-TOF-MS
- From:
Journal of Pharmaceutical Practice and Service
2025;43(11):548-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze chemical constituents of compound Maxing Shigan decoction by ultra-high perfor-mance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). Methods The separation was performed on a UPLC BEH C18 column (2.1 mm×100 mm, 2.5 µm)，with a gradient elution applying 0.1% aqueous formic acid solution and 0.1% formic acid acetonitrile as a mobile phase. The column temperature was 40 °C. The flow rate was 0.4 ml/min and the analysis time was 15 min. Mass spectrometry (MS) data were collected in both positive and negative ESI ion modes. Results Through UPLC-QTOF/MS analysis and reference validation, a total of 59 chemical components in Maxing Shigan decoction were identified. Conclusion An ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method was established to identify the chemical components of Maxing Shigan decoction. This method is simple, efficient, sensitive and accurate, and provides a basis for the elucidation of the pharmacodynamic material basis and mechanism of Maxing Shigan decoction. It can provide data reference for the optimization of the compatibility of traditional Chinese medicine in the treatment of COVID-19.
