Exogenous Hydrogen Sulfide Improves Hydrogen Peroxide-Induced Senescence of Human Umbilical Vein Endothelial Cells via PI3K/Akt/eNOS Pathway
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0408
- VernacularTitle:外源性硫化氢通过PI3K/Akt/eNOS通路改善过氧化氢诱导的人脐静脉内皮细胞衰老
- Author:
Hai-ming NIU
1
;
Jian-wei LI
1
;
Miao-lian CHEN
1
;
Ding-hui LIU
2
;
Lin WU
2
;
Ye-sheng LING
2
;
Xian-guan YU
2
;
Xiao-xian QIAN
2
Author Information
1. Department of Critical Care， Zhongshan People’s Hospital/Zhongshan Affiliated Hospital， Sun Yat-sen University， Zhongshan 528400， China
2. Department of Cardiology， The Third Affiliated Hospital， Sun Yat-sen University， Guangzhou 510630， China
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
human umbilical vein endothelial cells;
hydrogen peroxide;
senescence;
PI3K/Akt/eNOS signaling pathway
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(4):535-542
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of PI3K/Akt/eNOS signaling pathway in the protective effect of hydrogen sulfide（H2S） on endothelial cell senescence induced by hydrogen peroxide （H2O2）. MethodsSenescence in human umbilical vein endothelial cells （HUVEC） was induced by 60 μmol/L H2O2 for 1 h， and the effects of NaHS on the senescence were examined by the expression of plasminogen activator inhibitor 1 （PAI-1） and senescence-associated β-galactosidase （SA-β-Gal） staining. Meanwhile，the expression of protein kinase B（Akt）， Phospho protein kinase B（p-Akt）， and endothelial nitric oxide synthase （eNOS） were examined by western blot， and the senescence-related parameters. The content of the nitric oxide （NO） was measured by nitrate reduction method. ResultsCompared with the control group， after being treated with 60 μmol/L H2O2， the number of SA-β-gal positive cells and the expression of PAI-1 were significantly increased. However， after pretreatment of NaHS， the expression of PAI-1， the ratio of SA-β-gal positive cells was significantly decreased， but the expression of p-Akt， eNOS and the NO concentration increased. ConclusionNaHS could reverse HUVEC senescence induced by H2O2， and this may be highly associated with PI3K/Akt/eNOS signaling pathway.
