Characteristics of Intestinal Injury in Diabetic Mice with Sepsis
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0405
- VernacularTitle:糖尿病合并脓毒症小鼠肠损伤特点
- Author:
Chang-ji PANG
1
;
Fang TAN
1
;
Lei ZHENG
1
;
Yu-Ling CAO
1
;
Ying-qing DENG
1
;
Xin-jin CHI
1
Author Information
1. Department of Anesthesia，The Seventh Affiliated Hospital of Sun Yat-sen University，Shenzhen 518107，China.
- Publication Type:Journal Article
- Keywords:
diabetes;
sepsis;
intestinal injury;
macrophage polarization
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(4):513-520
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe present study was designed to explore the role of high glucose and macrophage polarization and their potential relation to septic intestinal injury. MethodsBKS-DB （Lepr ko/ko） and BKS-DB （Lepr wt/wt） male mice were randomly divided into 4 groups （n=6）， which include non-diabetic mice sham group （NDMS group）， non-diabetic mice CLP group （NDMCLP group）， diabetic mice sham group （DMS group）， diabetic mice CLP group （DMCLP group）. The cell experiment was divided into four groups： control group （Sham group）， high glucose group （HG group）， lipopolysaccharide group （LPS group）， high glucose and lipopolysaccharide group （HG+LPS group）. Animal and cell models were respectively established by cecal ligation and puncture （CLP） and co-stimulation of high glucose and LPS. The pathological injury and Occludin and ZO-1 protein expression of intestinal tissue were detected. The distribution and polarization characteristics of macrophages were detected by immunofluorescence， real-time quantitative PCR （RT-PCR） and Western blot. ResultsTwelve hours after CLP， the intestinal pathological scores significantly increased in the DMCLP group （P<0.05）. The results of western blot and RT-PCR showed the expressions of intestinal Occludin and ZO-1 protein were decreased in the DMCLP group， while the expression of inflammatory cytokines TNF-α， IL-1β and IL-6 mRNA was increased significantly （P<0.05）. The results of immunofluorescence and RT-PCR showed the number of macrophages in the intestinal tissue of mice in the DMCLP group increased significantly， and the expression of M1 markers iNOS and CRR7 mRNA increased significantly （P<0.05）. In further cell experiment， the expressions of M1 macrophages marker CD86 and inflammatory cytokine TNF-α， IL-1β and IL-6 mRNA were significantly increased in the HG+LPS group. ConclusionHigh glucose might aggravate intestinal injury by promoting LPS-induced macrophage polarization to M1 type.
