Combined NMR and HILIC-UPLC-MS/MS Analysis to Detect Metabolic Changes of Urine and Plasma from Rats with TNBS-induced Colitis
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0404
- VernacularTitle:NMR联用HILIC-UPLC-MS/MS测定TNBS诱导的结肠炎大鼠尿液和血浆代谢物变化
- Author:
Wen LIU
1
;
Yang-wen SUN
1
;
Bao-rong ZOU
1
;
Dan XIAO
1
;
Ying CAI
1
;
De-liang LIU
1
;
Qiong-feng LIAO
2
;
Zhi-yong XIE
1
Author Information
1. School of Pharmaceutical Sciences （Shenzhen）， Sun Yat-sen University， Shenzhen， 518107， China
2. School of Chinese Materia Medica， Guangzhou University of Chinese Medicine， Guangzhou， 510006， China
- Publication Type:Journal Article
- Keywords:
colitis;
metabolomics;
NMR;
HILIC-UPLC-MS/MS
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(4):504-512
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe aim of this study was to investigate the variations of endogenous metabolites in the urine and plasma of 2，4，6-trinitro benzene sulfonic acid （TNBS）-induced colitis model rats on the base of untargeted and targeted metabolomics techniques. MethodsA total of 16 male SD rats were randomly and equally divided into control group and model group. The rats in the model group were treated with TNBS enema to establish experimental colitis rat model. The urinary metabolites of rats in the both two groups were detected by employing 1H nuclear magnetic resonance （1H NMR）. The levels of amino acids in urine and plasma of above rats were investigated by hydrophilic interaction ultra-high-performance liquid chromatography-tandem mass spectrometry （HILIC-UPLC-MS/MS）. Multivariate statistical analysis was combined to analyze the metabolic profile additionally. ResultsCompared with the control group， the 1H NMR results showed that the metabolic profile of urine in the model rats was significantly changed， including increased levels of pyruvate， formate， methylamine and citrate， decreased levels of trimethylamine oxide and malonate （all P < 0.05）. Meanwhile， the HILIC-UPLC-MS/MS results indicated that the levels of phenylalanine and histidine were significantly increased in the urine of model rats， the contents of lysine， arginine， phenylalanine， leucine， glycine， tryptophan， proline， histidine and tyrosine were markedly elevated while glutamine， valine， alanine and isoleucine were notably reduced in the plasma of model rats （all P < 0.05）. ConclusionsThe metabolic pathways including energy metabolism， amine metabolism and amino acid metabolism were interfered in colitis rats induced by TNBS. The multivariate metabolomics methods based on 1H NMR and HILIC-UPLC-MS/MS revealed the metabolic alterations of urine and plasma in the colitis rats， providing new perspective for the mechanism research of inflammatory bowel disease （IBD） and the exploration of related biomarkers.
