Effects of EZH1/2 Inhibitor UNC1999 on Immunophenotypes of Peripheral Immune Cells
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0403
- VernacularTitle:EZH1/2抑制剂UNC1999对外周血免疫细胞表型的影响
- Author:
Dan-ping LIU
1
;
Pei-dong CHI
2
;
Mei-hua MEI
1
;
Ying YOU
1
;
Jun-qi HUANG
1
Author Information
1. Department of Transplantation， The First Affiliated Hospital//Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology//Guangdong Provincial International Cooperation Base of Science and Technology （Organ Transplantation）， Sun Yat-sen University， Guangdong 510080， China
2. Department of Clinical Laboratory， Sun Yat-Sen University Cancer Center， Guangzhou 510060， China
- Publication Type:Journal Article
- Keywords:
EZH1/2;
UNC1999;
multicolor flow cytometry;
immunophenotype
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2021;42(4):494-503
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of EZH1/2 inhibitor UNC1999 on the immune cell phenotypes in peripheral blood of healthy adults. MethodsCCK8 assay was used to measure the cell viability of peripheral blood mononuclear cells （PBMC）. Multicolor flow cytometry was performed to analyze the immunophenotypes. ResultsCompared with DMSO group， UNC1999 group showed increased classical monocytes （CD14++CD16-） ［（19.53±1.79）% vs. （66.60±5.02）%， t=13.31， P=0.006）， decreased intermediate monocytes （CD14++CD16+） and non-classical monocytes （CD14+CD16+） ［（35.08±3.97）% vs. （15.42±2.89）%， t=6.130， P=0.026； （35.50±3.53）% vs. （8.40±3.12）%， t=25.740， P=0.002］. The proportions of CD56dim CD16+， CD56dom CD16+ in UNC1999 group were lower ［（3.39±0.86）% vs. （0.27±0.06）%， t=4.882， P=0.040； （80.50±0.64）% vs. （0.63±0.23）%， t=133.100， P<0.000 1］. UNC1999 group exhibited higher frequency of naive B cells ［（10.67±1.76）% vs. （37.99±3.76）%， t=17.690， P=0.003］， lower frequency of memory B cells， transitional B cells， plasmablasts B cells ［（23.39±4.20）% vs. （11.82±1.90）%， t=7.059， P=0.020； （3.58±0.47）% vs. （1.52±0.56）%， t=26.970， P=0.001； （0.18±0.03）% vs. （0.00±0.00）%， t=8.647， P=0.013］. The percentage of DC and mDC/DC was significantly elevated in UNC1999 group ［（0.20±0.05）%vs.（1.38±0.13）%， t=16.500， P=0.004； （32.41±13.14）% vs. （60.87±8.43）%， t=8.252， P=0.014］， with a significantly decreased percentage of pDC/DC ［（24.90±1.95）% vs. （12.70±2.11）%， t=7.566， P=0.017］. Higher proportions of CD8+ central memory T cells （TCM） and CD8+PD-1+ ［（5.62±1.24）% vs. （18.38±2.34）%， t=15.600， P=0.004； （2.50±1.02）% vs. （18.34±2.69）%， t=8.822， P=0.013］， but lower proportions of CD8+ effective memory T cells （TEM） and CD4+CD27+were observed in UNC1999 ［（28.27±10.15）% vs. （15.62±9.48）%， t=19.480， P=0.003； （82.77±2.66）% vs. （56.00±9.01）%， t=5.715， P=0.029］. No statistical difference was found in other cell subsets （P>0.05）. ConclusionUNC1999 can lead to changes in PBMC immunophenotypes.
