Screening for chronic inflammation-related biomarkers in acute ischemic stroke and asthma based on the transcriptomic analysis
10.19845/j.cnki.zfysjjbzz.2025.0171
- VernacularTitle:基于转录组学分析的急性缺血性脑卒中与哮喘的慢性炎症相关的生物标志物筛选
- Author:
Fan YANG
1
Author Information
1. Department of Pharmacy，Nanjing Drum Tower Hospital，Nanjing 210000，China
- Publication Type:Journal Article
- Keywords:
Acute ischemic stroke;
Asthma;
Chronic inflammation;
ABCA2
- MeSH:
Asthma
- From:
Journal of Apoplexy and Nervous Diseases
2025;42(10):928-938
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore chronic inflammation-related biomarkers in acute ischemic stroke and asthma using bioinformatics techniques， and to provide molecular etiological evidence for preventing acute ischemic stroke in patients with asthma. Methods R package was used to analyze the transcriptomic data on acute ischemic stroke and asthma from GSE202518 and GSE207751 datasets， and the two datasets were analyzed using differential analysis， weighted gene co-expression network analysis （WGCNA）， gene ontology （GO） analysis， Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis，Gene Set Enrichment Analysis （GSEA）， and immune infiltration correlation analysis. Results The analysis of the two datasets showed that 74 overlapping differentially expressed genes （DEGs） were significantly upregulated in both acute ischemic stroke patients and asthma patients. The GO， KEGG， and GSEA analyses showed that changes in neutrophil-related biological processes were significantly associated with both acute ischemic stroke and asthma. The integration of WGCNA key modules and DEGs showed that ABCA2 was a critical gene that could influence both diseases. The immune infiltration analysis further revealed that the numbers of memory B cells， naïve CD4+ T cells， activated NK cells， and resting mast cells were negatively correlated with the expression of ABCA2， while follicular helper T cells was positively correlated with the expression of ABCA2. Conclusion ABCA2 is highly expressed in both acute ischemic stroke patients and asthma patients， and it serves as a key biomarker associated with chronic inflammation and plays an important role in the progression of asthma comorbid with acute ischemic stroke. ABCA2 may provide a novel target for further mechanism research and offer new strategies for preventing acute ischemic stroke in patients with asthma.
