Establishment of a modified neurovascular unit model in vitro
10.19845/j.cnki.zfysjjbzz.2025.0189
- VernacularTitle:改良型体外神经血管单元模型构建
- Author:
Chuan CAI
1
;
Xiaofan CHU
2
Author Information
1. 北京大学深圳医院神经内科， 广东 深圳 518036
2. 暨南大学第二临床医学院， 广东 深圳 518020
- Publication Type:Journal Article
- Keywords:
Neurovascular unit;
Cerebral infarction;
Ischemia-reperfusion injury;
Co-culture system
- From:
Journal of Apoplexy and Nervous Diseases
2025;42(11):1034-1037
- CountryChina
- Language:Chinese
-
Abstract:
Objective There are complex pathophysiological changes in each component of cerebral tissue after cerebral ischemia-reperfusion injury，and routine neuroprotective strategies fail to exert an effective therapeutic effect in clinical practice. However， the proposal of neurovascular unit（NVU） provides a new conceptual framework for a more comprehensive understanding of the pathophysiological process for interactions between each component of cerebral tissue after cerebral ischemia-reperfusion injury， laying a theoretical foundation for investigating new targets for clinical treatment. Methods A novel NVU in vitro model was established in Transwell co-culture plate using the neurons， astrocytes， and brain microvascular endothelial cells （BMECs） derived from the cortex of Sprague-Dawley rats， and MTT assay was used to measure cell viability in the modified and classical NVU models after OGD/R injury for 1 hour. Results An in vitro NVU model with the three-layer structure of neurons， astrocytes， and BMECs from the top to the bottom was successfully established using Transwell co-culture plate. After OGD/R injury for 1 hour， the neurons in the modified in vitro NVU model showed a significantly higher viability than those in the classical NVU model （P<0.05）， while there were no significant differences in the viability of astrocytes and BMECs between the two models （P>0.05）. Conclusion The modified in vitro NVU model is more suitable for the research on ischemic cerebrovascular disease.
