Determination of 6PPDQ in human plasma and urine by ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry
10.20001/j.issn.2095-2619.20250814
- VernacularTitle:超高效液相色谱-串联三重四极杆质谱检测人血浆和尿中6PPDQ
- Author:
Wenjie ZHANG
1
;
Jing YUAN
;
Anping MA
;
Jingjing QIU
;
Yukun CHEN
;
Yiru QIN
;
Banghua WU
Author Information
1. School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong 510080, China
- Publication Type:Journal Article
- Keywords:
Plasma;
Urine;
6PPDQ;
Ultra-high-performance liquid chromatography;
Triple quadrupole mass spectrometry;
Detection
- From:
China Occupational Medicine
2025;52(4):441-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry method for the determination of N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine quinone (6PPDQ) in human plasma and urine. Methods Plasma and urine samples (0.3 mL each) were mixed with 0.9 mL acetonitrile and dichloromethane, vortexed, and subjected to ultrasonic treatment to facilitate extraction. After centrifugation, the extract was collected, evaporated to dry powder under nitrogen, and reconstituted. Separation was performed on a C18 column, and detection was carried out using ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry with external standard quantification. Results 6PPDQ showed good linearity in the range of 0.01-25.00 μg/L in both human plasma and urine, with correlation coefficients of 0.999 5 and 0.999 7, respectively. The detection limits for plasma and urine were 8 and 6 ng/L, and the lower limits of quantification were 27 and 19 ng/L, respectively. The average recovery rates were 97.00%-100.00% for plasma and 90.00%-96.50% for urine. The within-run relative standard deviations (RSDs) were 4.35%-10.00% for plasma and 2.34%-11.11% for urine, while the between-run RSDs were 6.80%-8.46% and 2.60%-10.00%, respectively. Samples can be stored for seven days at 4 ℃ or -20 ℃. respectively. Samples can be stored for seven days at 4 ℃ or -20 ℃. Matrix effects ranged from 87.12%-99.27% for plasma and 91.00%-97.56% for urine. Conclusion The proposed method is simple, highly sensitive, and reproducible, and is suitable for the determination of 6PPDQ in human plasma and urine samples.
