Serological detection of anti-Mur and the distribution of the Mur antigen among voluntary blood donors
10.13303/j.cjbt.issn.1004-549x.2025.10.016
- VernacularTitle:抗-Mur的血清学检测及在无偿献血者Mur血型分布的应用研究
- Author:
Qunfeng SHU
1
;
Ji ZHOU
1
;
Huan ZHAO
2
;
Dong LIU
1
;
Dongju PENG
1
;
Zhiping YANG
1
;
Yingying TANG
3
Author Information
1. Shiyan Central Blood Station, Shiyan 442000, China
2. Shiyan Hospital of Integrated Traditional Chinese and Western Medicine, Shiyan 442000, China
3. Zhuxi County People's Hospital, Shiyan 442300, China
- Publication Type:Journal Article
- Keywords:
Mur antigen;
anti-Mur;
unexpected antibody;
Fisher's exact test
- From:
Chinese Journal of Blood Transfusion
2025;38(10):1403-1407
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the serological characteristics of anti-Mur antibodies and investigate the distribution frequency of the Mur antigen among voluntary blood donors in Shiyan, thereby providing a basis for guiding clinical transfusion and establishing a Mur blood type database. Methods: ABO blood grouping of donors and patients was performed using an automated blood typing analyzer and the gel card method, respectively. Unexpected antibody screening and identification were performed using the saline, tube anti-human globulin, and polybrene methods. The specificity of anti-Mur antibodies was confirmed using Fisher's exact probability test. Plasma treated with 2-mercaptoethanol was used to distinguish IgM and IgG antibodies. IgM and IgG anti-Mur titers were determined by the saline tube method and the anti-human globulin tube method, respectively, at 4℃, room temperature, and 37℃. A total of 1 659 donor red blood cell samples were initially screened for the Mur antigen phenotype using three samples of human-derived anti-Mur plasma by the micro-tube method. Donors who tested positive for Mur antigen were further tested by the direct antiglobulin test (DAT); those with negative results were confirmed for Mur antigen by the gel card and polybrene methods. Results: Three blood samples were identified to contain mixed IgG and IgM anti-Mur antibodies. The titers of both IgM and IgG anti-Mur antibodies were highest at 4℃, intermediate at room temperature, and lowest at 37℃. The positive frequency of the Mur antigen among voluntary blood donors in Shiyan was 1.99% (33/1 659). Conclusion: anti-Mur antibodies were detected in both blood donors and patients in our region. The Mur antigen shows a certain distribution frequency among voluntary blood donors in Shiyan. Screening for the Mur blood type and establishing a corresponding database could enhance transfusion safety.
