Characterization of hemolytic transfusion reactions induced by anti-E antibodies
10.13303/j.cjbt.issn.1004-549x.2025.11.016
- VernacularTitle:抗-E导致溶血性输血反应的特征研究并文献复习
- Author:
Huali HUANG
1
;
Hao LI
1
;
Yuerong WEI
1
;
Ruixian LUO
1
;
Huini HUANG
1
;
Huiqiong XIE
1
;
Hailan LI
1
;
Ziji YANG
1
;
Zhuning MO
1
Author Information
1. Department of Blood Transfusion, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
- Publication Type:Journal Article
- Keywords:
anti-E;
enzyme-only antibody;
transfusion reaction;
Rh blood group;
alloantibody
- From:
Chinese Journal of Blood Transfusion
2025;38(11):1577-1585
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the causes and characteristics of immune hemolytic transfusion reactions (HTRs) triggered by anti-E antibodies, so as to provide a scientific reference for guaranteeing clinical blood transfusion safety. Methods: Five patients who experienced HTRs in our hospital from November 2023 to October 2024 were selected as the research subjects. ABO/RhD blood grouping, antibody screening, antibody identification, and the direct antiglobulin test (DAT) were conducted using the column agglutination method. The causes of HTRs in these patients were investigated using multiple techniques such as the two-step enzyme method, polyethylene glycol (PEG), acid elution technique, and capillary centrifugation method. Results: All five patients tested negative for antibody screening prior to transfusion. However, after transfusion of E+ phenotyped blood, patients 1, 2, 3, and 5 developed delayed haemolytic transfusion reaction (DHTR), while patient 4 experienced acute haemolytic transfusion reaction (AHTR). Anti-E antibodies were detected in all blood samples from the patients after the hemolytic transfusion reaction, including the enzyme-only anti-E antibody in two cases. Conclusion: Anti-E antibody can trigger both intravascular and extravascular hemolysis. It is recommended to conduct ABO/RhD and RhE antigen-matched transfusions and establish a regional blood transfusion database to reduce immune hemolytic transfusion reactions caused by anti-E antibody.
