Investigation on the management of hemolytic and lipemic samples in the preanalytical phase in blood station laboratories in North China
10.13303/j.cjbt.issn.1004-549x.2025.11.008
- VernacularTitle:华北地区血站实验室检测前溶血和乳糜血标本管理情况调查
- Author:
Jing SUN
1
;
Hongwei GE
1
;
Zhengmin LIU
1
;
Qianqian QIN
1
;
Wei HAN
2
;
Tong PAN
3
;
Dongli JIAO
4
;
Xiaolan DONG
5
;
Rui WANG
1
Author Information
1. Beijing Red Cross Blood Center, Beijing 100088, China
2. Hebei Blood Center, Shijiazhuang 050000, China
3. Tianjin Blood Center, Tianjin 300074, China
4. Shanxi Blood Center, Taiyuan 030012, China
5. Inner Mongolia Autonomous Region Blood Center, Huhehot 010050, China
- Publication Type:Journal Article
- Keywords:
hemolytic blood;
lipemic blood;
the North China region;
preanalytical process
- From:
Chinese Journal of Blood Transfusion
2025;38(11):1529-1534
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the assessment criteria and subsequent handling practices of hemolytic and lipemic blood samples before testing in blood screening laboratories in North China, and to provide data to support the standardization of their management in blood station laboratories. Methods: Data on the preanalytical management of hemolytic and lipemic samples from 38 laboratories were collected. The details of management on the criteria and verificatioon for assessment, the assessment methods, and subsequent handling procedures of hemolytic and lipemic samples in blood station laboratories were analyzed. Results: 1) All 38 blood station laboratories monitored serological and nucleic acid samples for hemolysis and lipemia in pre-analytical phase. 2) The criteria and methods for assessing hemolytic and lipemic samples varied among the laboratories of the 38 blood stations. 15 laboratories (39.47%) followed manufacturer's instructions, 9 laboratories (23.68%) formulated their own criteria, and 14 laboratories (36.84%) referred to the criteria of other laboratories. 16 laboratories (42.11%) verified the criteria for assessing hemolytic and lipemic samples, with significant variations in verification rate across laboratories from different regions (P<0.05). For the assessment methods, visual inspection was used by 28 laboratories (73.68%) for hemolytic samples and by 27 laboratories (71.05%) for lipemic samples; the colorimetric card method was used by 10 laboratories (26.32%) for assessing both hemolytic and lipemic samples; the instrumental method was used by 1 laboratory (2.63%) for assessing lipemic samples.3) The handling procedures for hemolytic and lipemic samples varied significantly and followed a gradient distribution pattern among 38 laboratories (including accepting samples for testing, accepting samples for concession testing, re-collecting samples, and rejecting samples and halting testing). With increasing severity of hemolysis and lipemia, more laboratories halted testing, and relatively fewer laboratories accepted samples for normal testing. 5 laboratories (13.16%) applied different handling procedures on serological and nucleic acid samples. Conclusion: This survey provides a reference and basis for analyzing gaps in the management of hemolytic and lipemic samples during the preanalyical phase in blood station laboratories in North China. It enables laboratories to identify the problems and deficiencies in the management of hemolytic and lipemic samples, to ensure preanalytical samples quality meets the established requirements, and to lay a foundation for promoting the homogenization and standardization of the regional sample quality management mode.
