Effect of Exosomes CXCL1 on the Proliferation,Invasion,and Migration of Cervical Cancer Cells
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2025.0612
- VernacularTitle:外泌体CXCL1对宫颈癌细胞增殖、侵袭及迁移的影响
- Author:
Fengmei ZHANG
1
;
Yanan ZUO
;
Juncheng ZHANG
;
Qianqian HU
;
Hongfang LI
Author Information
1. 甘肃中医药大学第一临床医学院,甘肃 兰州 730000;兰州市第一人民医院妇产科,甘肃 兰州 730000
- Publication Type:Journal Article
- Keywords:
cervical cancer;
exosomes;
C-X-C chemokine ligand 1;
proliferation;
invasion;
migration
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(6):1021-1028
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the effects of exosomal CXCL on the biological behavior of cervical cancer cells and its underlying mechanisms.[Methods]Chemokine CXCL was first screened through bioinformatics databases.The GEPIA database was analyze CXCL expression in cervical cancer tissues and adjacent normal cervical tissues.Western blot was performed to detect CXCL expression levels in cervical cancer cells(Caski)and normal cervical epithelial cells(H8).The successful isolation of exosomes was confirmed by nanoparticle tracking analysis,transmission electron microscopy(TEM)and Western blot.ELISA was employed to detect the expression level of exosomes CXCL was determined by ELISA.After CXCL knockdown via siRNA transfection,cells were divided into three groups:blank control,negative control and experimental groups.Cell proliferation was evaluated using the CCK-8 assay,while cell migration and invasion were assessed by Transwell assays.[Results]Exosomal CXCL expression was significantly upregulated in cervical cancer cells compared with normal cervical epithelial cells(P<0.01),and also markedly elevated in cervical cancer tissues compared with adjacent normal tissues.After low expression of CXCL knockdown significantly reduced CXCL expression in both cancer cells and their derived exosomes(P<0.05).Low expression markedly inhibited the proliferation,invasion and migration abilities[Conclusion]Silencing exosomal CXCL may inhibit the malignant biological behavior of cancer cells.
