Effect of Cordyceps Sinensis Extract in Antidrug Resistance of Immunotherapy for Lung Cancer
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20251112.001
- VernacularTitle:冬虫夏草提取物在克服肺癌免疫治疗耐药中的作用
- Author:
Minyuan LU
1
;
Ruonan WANG
2
;
Xiaolei ZHANG
1
;
Xiaofan MA
3
Author Information
1. School of Pharmacy， Sun Yat-sen University， Guangzhou 510006， China
2. Haide College， Ocean University of China， Qingdao 266003， China
3. Sun Yat-sen Memorial Hospital， Sun Yat-sen University， Guangzhou 510120， China
- Publication Type:Journal Article
- Keywords:
Cordyceps sinensis;
non-small cell lung cancer;
immunotherapy;
drug resistance;
anti-cancer
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2025;46(6):1015-1020
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the reversal effect of Cordyceps sinensis extract on immunotherapy resistance of non-small cell lung cancer （NSCLC）. MethodsELISA， flow cytometry， and co-culture assays were performed to evaluate the effects of Cordyceps sinensis extract on cytokine secretion， proliferation， and tumor cell killing activity of Jurkat （JKT） cells. Furthermore， the therapeutic effect of Cordyceps sinensis extract on programmed cell death ligand 1 （PD-L1） resistant lung cancer was evaluated through in vivo experiments both alone and in combination with anti-PD-L1 monoclonal antibody. ResultsCordyceps sinensis extract enhanced the secretion of cytokines interferon gamma（IFN-γ）， tumor necrosis factor-alpha（TNF-α） and interleukin-2 （IL-2）in JKT cells， promoted cell proliferation （P=0.006）， and boosted their killing function against tumors （P＜0.001）. Compared with the control group， in vivo study demonstrated that Cordyceps sinensis extract monotherapy effectively inhibited the growth of PD-L1-resistant tumors ［tumor growth inhibition value （TGI）=26.1%， P=0.090］， while its combination with anti-PD-L1 antibody significantly reversed PD-L1 resistance （TGI=50.6%， P＜0.001）. ConclusionThis study provides certain data support for the activation of anti-tumor immunity by Cordyceps sinensis extract， and offers a new treatment strategy for enhancing anti-tumor immunity and reversing immune resistance in lung cancer.
