Study on the effect of apoptosis stimulation protein 2 on traumatic proliferative vitreoretinopathy in rabbits
10.3980/j.issn.1672-5123.2026.1.03
- VernacularTitle:ASPP2对兔外伤性增生性玻璃体视网膜病变的作用研究
- Author:
Xiaoli CHEN
1
;
Yuze MAO
1
;
Wenhui CAI
1
;
Haiwei WANG
1
;
Yankun YUE
1
Author Information
1. Department of Ophthalmology, Fu Xing Hospital, Capital Medical University, Beijing 100038, China
- Publication Type:Journal Article
- Keywords:
proliferative vitreoretinopathy;
apoptosis stimulation protein 2(ASPP2);
animal model;
traumatic;
retinal pigment epithelial cells
- From:
International Eye Science
2026;26(1):16-20
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of apoptosis stimulation protein 2(ASPP2)on the development of traumatic proliferative vitreoretinopathy(PVR)in a rabbit model.METHODS:A total of 30 New Zealand white rabbits were selected, and the right eyes of all rabbits were inflicted with a scleral penetrating wound of approximately 6 mm. Then rabbits were randomly and evenly divided into experimental and control group. The experimental group received an intravitreal injection of 0.1 mL of ARPE-19 cell suspension transfected with lentivirus-ASPP2, while the control group received an intravitreal injection of 0.1 mL of ARPE-19 cell suspension transfected with negative control lentivirus. At 1, 2, 3, and 4 wk after PVR modeling, a handheld tonometer was used to measure the intraocular pressure. Moreover, fundus photography and ocular ultrasound examination were performed to detect the retinal proliferation. At 4 wk after modeling, hematoxylin-eosin staining was used to observe the morphological retinal changes, and Western blot was used to determine the protein expressions of ASPP2 and the epithelial-mesenchymal transition(EMT)marker Vimentin in the rabbit retinas.RESULTS:At 1, 2, 3, and 4 wk after modeling, there were no significant changes in intraocular pressure within the experimental and control group of rabbit eyes, either before or after PVR modeling, the success rate of PVR modeling in the experimental group was lower than that in the control group(P<0.05), and the retinal proliferation and structural disorder was less severe in the experimental group. At 4 wk after modeling, the retinal protein expression level of ASPP2 in the experimental group was significantly higher than that in the control group(t=3.193, P=0.033), while the Vimentin protein expression level was significantly lower in the experimental group(t=-3.599, P=0.023).CONCLUSION:ASPP2 may be involved in regulating the process of EMT in retinal pigment epithelial cells, thereby delaying the development and progression of traumatic PVR in rabbit eyes.
