Identification and Biological Characterization of Pathogen and Screening of Effective Fungicides for Wilt of Tetradium ruticarpum
10.13422/j.cnki.syfjx.20251615
- VernacularTitle:吴茱萸枯萎病病原菌鉴定、生物学特性及其有效杀菌剂筛选
- Author:
Yuxin LIU
1
;
Qin XU
1
;
Yue YUAN
1
;
Tiantian GUO
1
;
Zheng'en XIAO
1
;
Shaotian ZHANG
1
;
Ming LIU
1
;
Fuqiang YIN
1
Author Information
1. College of Biology and Food Engineering， Chongqing Three Gorges University， Chongqing 404120，China
- Publication Type:Journal Article
- Keywords:
Tetradium ruticarpum;
Fusarium fujikuroi;
wilt;
biological characteristics;
fungicide screening
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(2):198-206
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the pathogen species responsible for the wilt disease of Tetradium ruticarpum in Chongqing， investigate there biological characteristics， and screen effective fungicides， so as to provide a theoretical basis for disease control in production. MethodsThe pathogen was isolated via the tissue culture method. Pathogenicity was verified according to Koch's postulates. The pathogen was identified based on morphological characteristics and multi-gene phylogenetic analysis. The mycelial growth rate method was used for biological characterization of the pathogen and fungicide screening. ResultsThe pathogen colonies were nearly circular with irregular edges， white， short， velvety aerial hyphae， and pale purple undersides. Macroconidia were colorless， sickle-shaped， with 3-5 septa， while microconidia were transparent， elliptical， aseptate or with 1-2 septa. Multi-gene phylogenetic analysis showed that the pathogen clustered in the same clade as Fusarium fujikuroi with 100% support， which， combined with morphological characteristics， identified the pathogen causing wilt of T. ruticarpum in Chongqing as F. fujikuroi. The optimal conditions for the mycelial growth of F. fujikuroi were mung bean agar （MBA） with glucose as the carbon source， beef extract and yeast powder as nitrogen sources， 28 ℃， pH 7.0， and alternating light/dark conditions. The optimal conditions for sporulation were potato dextrose agar （PDA） with glucose as the carbon source， beef extract as the nitrogen source， 28 ℃， pH 7.0， and complete darkness. Among chemical fungicides， phenazine-1-carboxylic acid exhibited the strongest inhibitory effect on F. fujikuroi. Shenqinmycin and tetramycin were the most effective bio-fungicides. ConclusionThis study is the first to report F. fujikuroi as the causal agent of wilt disease in T. rutaecarpa. The chemical fungicide phenazine-1-carboxylic acid and the bio-fungicides shenqinmycin and tetramycin showed strong inhibitory effects against F. fujikuroi.
