Establishment and comparison of infection models of Bordetella pertussis aerosol and intratracheal nebulization in mice
10.13200/j.cnki.cjb.004609
- VernacularTitle:百日咳杆菌小鼠气溶胶与气管内雾化感染模型的建立及比较
- Author:
Fen WANG
1
Author Information
1. Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center for Combined Vaccines, Hubei Vaccine Technology Innovation Center, Wuhan 430207, Hubei Province, China
- Publication Type:Journal Article
- Keywords:
Pertussis;
Mouse model;
Aerosol;
Intratracheal nebulization
- From:
Chinese Journal of Biologicals
2025;38(12):1416-1421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish two models of pertussis infection in mice through aerosol and intratracheal nebulization,and compare and evaluate the infection effects.Methods Using fermale NIH mice with five for each group, the aerosol infection model was set up with 10~8, 10~9, 10~(10)CFU/mL and initial concentration(1. 42 × 10~(11)CFU/mL) groups, and the infection was carried out by nebulization inhalation in a closed space for 30 minutes. Intratracheal nebulization infection model adopted a fixed nebulization dose of 25 ??L, including 10~3, 10~4 and 10~5 CFU/25 ??L infected bacteria groups, by inserting into the trachea for nebulization infection. On the 3 rd, 7 th, 14 th, 21 st and 28 th days after infection, three mice in each group were selected for lung specific gravity detection, and one mouse was randomly selected for pathological section analysis of lung tissue. Finally, the mice were infected by aerosol or intratracheal nebulization, and observed for 14 days to calculate the half lethal dose(LD_(50)).Results The lung specific gravity of mice significantly increased when aerosol infection occurred at a bacterial concentration of 10~(10)CFU/mL, or when nebulization infection occurred in the trachea with a bacterial count of 10~4 CFU/25 ??L or above. Lung slices showed that intratracheal nebulization infection caused more significant pulmonary inflammatory infiltration than aerosol infection, and was more correlated with infection time and infection dose.The LD_(50)of intratracheal nebulization infection was 1. 88 × 10~8 CFU/25 ??L, while the aerosol infection did not reach the lethal dose.Conclusion Both respiratory tract infections can successfully establish a pertussis mouse infection model. The intratracheal nebulization infection model was more relevant to the dose and time of infection, and can be used as a lethal model, which has the potential to detect the potency of pertussis vaccine.
