Effect of different conditions of solid phase extraction column on determination of morphine in compound liquorice oral solution
10.12206/j.issn.2097-2024.202311012
- VernacularTitle:不同使用条件下固相萃取柱对复方甘草口服溶液吗啡含量测定的影响
- Author:
Linlin LIU
1
;
Guixiang ZHONG
1
;
Jia YAN
1
;
Xin ZHOU
1
Author Information
1. 900th Hospital of PLA Joint Logistic Support Force，Fuzhou 350025，China.
- Publication Type:Originalarticles
- Keywords:
solid phase extraction column;
compound liquorice oral solution;
morphine
- From:
Journal of Pharmaceutical Practice and Service
2025;43(12):599-602
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of different packing contents of solid phase extraction column, repeated use times and solid phase extraction column under pressure on the determination of morphine content in compound liquorice oral solution. Methods The component was performed by high performance liquid chromatography （HPLC） equipped with the C8 （4.6 mm × 150 mm, 5 μm） chromatographic column. The mobile phase consisted of acetonitrile-2.5 mmol/L sodium heptanesulfonate solution-50 mmol/L potassium dihydrogen phosphate solution （5∶18∶18）. The flow rate was 1.0 ml/min with the 220 nm detection wavelength, 20 μl injection volume and 30℃ column temperature. Results It was found that the SPE column produced of 500 mg had a higher recovery rate of morphine, and the RSD value was small. It was found that SPE column could be reused for up to 3 times under the condition of guaranteeing recovery rate.In the pressurized experiment,at the flow rate of 0.5 ml/min, the measured morphine content was basically consistent with that measured at atmospheric pressure. Conclusion The determination of morphine in compound liquorice oral solution could be carried out by C18 solid phase extraction column of 500 mg. Under pressure, the flow rate was about 0.5 ml/min for morphine recovery, which could achieve high recovery of morphine in compound liquorice oral solution and improve the detection efficiency of morphine.
