Advances in Construction and Application of Biliary Organoids.
10.3881/j.issn.1000-503X.16367
- Author:
Zhong-Wen LEI
1
;
Yang XIANG
2
;
Yi-Jun YANG
1
Author Information
1. Department of Hepatobiliary Surgery,Haikou Affiliated Hospital of Central South University Xiangya School of Medicine,Haikou 570208,China.
2. Haikou Key Laboratory of Clinical Research and Transformation of Digestive Diseases,Haikou 570208,China.
- Publication Type:Review
- Keywords:
biliary tract disease;
drug screening;
organoid;
regenerative medicine
- MeSH:
Organoids;
Humans;
Tissue Engineering/methods*;
Biliary Tract/cytology*
- From:
Acta Academiae Medicinae Sinicae
2025;47(4):611-620
- CountryChina
- Language:English
-
Abstract:
Biliary duct injury,congenital biliary atresia,biliary tract tumors,primary sclerosing cholangitis,etc.are common and refractory diseases in the digestive system in clinical practice.The existing surgical operations and drug treatments demonstrate limited effects.Organoids,as an emerging technology,have attracted much attention in recent years for deeply understanding the pathogenesis and development of these diseases and seeking more effective treatment approaches.An organoid,a three-dimensional complex derived from stem/progenitor cells,can simulate the complex structure and physiological function of tissues or organs in vitro.It provides an important platform for studying the pathogenesis of biliary tract diseases and brings new hope for the repair and regeneration of biliary tract injury.The seed cells for constructing biliary organoids are mainly biliary tract epithelial cells,pluripotent stem cells,etc.The conventional technologies for constructing biliary organoids mainly include embedding,rotary culture,and hanging drop culture.In recent years,new culture technologies such as organ chip and three-dimensional and four-dimensional printing are emerging.This article reviews the construction methods of biliary organoids,discusses the application of these organoids in disease model construction,disease mechanism research,drug screening,and tissue/organ repair,and proposes the current problems and future research directions of biliary organoids,which will provide reference for treating common refractory digestive system diseases in clinical practice.
