Screening of soil biocontrol bacteria and evaluation of their control effects on <i>Fusarium</i> head blight of wheat.

Dongfang WANG; Xinxin ZHAI; Chunlin YANG; Huilan ZHANG; Jie WU; Zerong SONG; Pan ZHAO; Yu CHI

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Screening of soil biocontrol bacteria and evaluation of their control effects on Fusarium head blight of wheat.

Author: Dongfang WANG ¹ ; Xinxin ZHAI ¹ ; Chunlin YANG ² ; Huilan ZHANG ³ ; Jie WU ¹ ; Zerong SONG ¹ ; Pan ZHAO ⁴ ; Yu CHI ⁵
Author Information

1. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China.
2. School of Plant Protection, Anhui Agricultural University, Hefei 230036, Anhui, China.
3. Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China.
4. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
5. Anhui Province Key Laboratory of Pesticide Resistance Management on Grain and Vegetable Pests, Hefei 230001, Anhui, China.
Publication Type:Journal Article
Keywords: Fusarium head blight of wheat; Pythium aphanidermatum; biocontrol; coleoptile experiment
MeSH: Triticum/growth & development*; Fusarium/growth & development*; Plant Diseases/prevention & control*; Soil Microbiology; Pest Control, Biological/methods*; Pythium/physiology*; Biological Control Agents; Rhizosphere; Fungicides, Industrial
From: Chinese Journal of Biotechnology 2025;41(10):3764-3773
CountryChina
Language:Chinese
Abstract: Fusarium head blight (FHB), caused by Fusarium graminearum, not only leads to severe yield losses but also poses a threat to food safety due to the mycotoxins produced by the pathogen. Since this disease is preventable but not curable, the current control mainly relies on chemical fungicides, the long-term use of which may lead to pathogen resistance and environmental pollution. To develop green control methods, we screened 13 biocontrol strains from the rhizosphere soil of wheat, among which strain No. 12 (identified as Pythium aphanidermatum) showed significant antifungal effects. In the plate confrontation test, this strain reduced the colony diameter of the pathogen by 69.2% (1.47 mm vs. 4.78 mm in the control group), with an inhibition rate of 77% (P < 0.01). Microscopic observation revealed obvious deformations in the pathogen hyphae, suggesting a lysing effect. The coleoptile experiment further confirmed that the pre-treatment with this strain reduced the incidence rate to 0. These findings provide new candidate strains for the biocontrol of FHB and offer a scientific basis for reducing the use of chemical fungicides and promoting sustainable agricultural development.