Screening and fermentation condition optimization of <i>Streptomyces scabies</i> antagonists.

Mengyan DOU; Ziwei WANG; Pan ZHAO; Xiu WANG; Aiping WANG; Naiqin ZHONG

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Screening and fermentation condition optimization of Streptomyces scabies antagonists.

Author: Mengyan DOU ¹ ; Ziwei WANG ² ; Pan ZHAO ³ ; Xiu WANG ⁴ ; Aiping WANG ¹ ; Naiqin ZHONG ³
Author Information

1. College of Agriculture, Shanxi Agricultural University, Jinzhong 030600, Shanxi, China.
2. College of Plant Protection, Shanxi Agricultural University, Jinzhong 030600, Shanxi, China.
3. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
4. Hulunbuir Agricultural and Animal Husbandry Technology Extension Center, Hulun Buir 021008, Inner Mongolia, China.
Publication Type:Journal Article
Keywords: Streptomyces scabies; antagonist; broad-spectrum resistance; fermentation conditions; potato scab
MeSH: Streptomyces/metabolism*; Fermentation; Plant Diseases/prevention & control*; Solanum tuberosum/growth & development*; Bacillus/growth & development*; Antibiosis
From: Chinese Journal of Biotechnology 2025;41(10):3747-3763
CountryChina
Language:Chinese
Abstract: In recent years, potato scab caused by Streptomyces scabies is aggravating year by year, becoming an industrial problem urgently to be resolved. Screening antagonistic bacteria with good inhibitory effect and wide adaptability is the main measure to realize effective prevention and control of the disease. This study screened three strains of antagonistic bacteria DXT2-4, T2-1 and 21-14 with good inhibitory effect on S. scabies by using plate standoff test, and identified them as Bacillus altitudinis, Bacillus safensis and Bacillus pumilus, respectively, based on morphological characteristics, physiological and biochemical properties, and 16S rRNA gene sequences. DXT2-4, T2-1 and 21-14 showed the pot control efficacy of 68.83%, 48.57%, and 57.14%, respectively. The field control efficacy of the three strains was 59.48%, 34.58% and 51.75% in Hulun Buir, Inner Mongolia Autonomous Region and 55.14%, 36.05%, and 49.05% in Huizhou, Guangdong. The three strains could grow normally in the media with pH 1.0-13.0 and with 1%-11% NaCl, and they had inhibitory effects on Rhizoctonia solani, Verticillium dahliae, Alternaria solani, and Fusarium oxysporum. The indole-3-acetic acid yields of DXT2-4, T2-1, and 21-14 were 2.23, 1.11, and 1.67 mg/L, respectively. DXT2-4 and 21-14 demonstrated strong abilities to solubilize phosphorus. The optimal carbon source, nitrogen source, and inorganic salt for fermentation of strain DXT2-4 were 2% molasses+2% corn starch, 2% soybean meal, and 0.3% MgSO₄·7H₂O, respectively. These findings suggest the three strains of bacteria can efficiently inhibit the growth of S. scabies and have strong environmental adaptability. Particularly, DXT2-4 has the best effects of inhibiting the disease and promoting plant growth, showing a high development value and broad application prospects, this is of great significance for promoting sustainable potato production and ensuring the environmentally sound utilization of resources.