Progress in the heterologous synthesis of sesquiterpenes by <i>Yarrowia lipolytica</i>.

Liuhuan WANG; Yuyao SHAO; Xiaolin CUI; Jiake YANG; Yan QIAO; Longxing XU; Yuejia SUN; Pengcai LIU; Lijie YUAN; Shuncheng LIU

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Progress in the heterologous synthesis of sesquiterpenes by Yarrowia lipolytica.

Author: Liuhuan WANG ¹ ; Yuyao SHAO ¹ ; Xiaolin CUI ¹ ; Jiake YANG ¹ ; Yan QIAO ¹ ; Longxing XU ¹ ; Yuejia SUN ² ; Pengcai LIU ¹ ; Lijie YUAN ¹ ; Shuncheng LIU ¹
Author Information

1. Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei, China.
2. College of Nursing and Rehabilitation, North China University of Science and Technology, Tangshan 063210, Hebei, China.
Publication Type:English Abstract
Keywords: Yarrowia lipolytica; amorphadiene; synthetic biology; α-farnesene; α-humulene; β-elemene; β-farnesene
MeSH: Yarrowia/genetics*; Sesquiterpenes/metabolism*; Metabolic Engineering/methods*; Synthetic Biology/methods*
From: Chinese Journal of Biotechnology 2025;41(9):3426-3447
CountryChina
Language:Chinese
Abstract: Sesquiterpenes are natural terpenes containing 15 carbon atoms. They are widely used in the perfume, pharmaceutical, and biofuel industries due to their remarkable biological activities. The traditional production of sesquiterpenes relies on chemical synthesis or plant extraction, which has the disadvantages of low yields and waste of resources. The construction of microbial cell factories for the efficient synthesis of sesquiterpenes by means of synthetic biology provides a new option. In recent years, with the development of metabolic engineering and synthetic biology, the heterologous synthesis of a variety of sesquiterpenes has been successfully achieved by metabolic engineering of the oleaginous yeast, Yarrowia lipolytica. In this paper, we review the research progress in the heterologous synthesis of different sesquiterpenes by Y. lipolytica, discuss the synthetic biology strategies commonly used in this field, and make an outlook on the research directions and engineering approaches to further enhance the sesquiterpene yield in this host. This paper provides a reference for strategies such as synergistic optimization of synthetic biology and metabolic engineering, enhanced precursors, and opens up new directions for the application of synthetic biology in green chemistry and sustainable production.