High-throughput screening technologies in the engineering of actinomycete strains.

Xueyan LIU; Meng WANG; Jifeng LIU; Yue ZHANG

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High-throughput screening technologies in the engineering of actinomycete strains.

Author: Xueyan LIU ¹ ; Meng WANG ² ; Jifeng LIU ¹ ; Yue ZHANG ²
Author Information

1. College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
2. State Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Publication Type:English Abstract
Keywords: actinomycete; fluorescence-activated cell sorting; fluorescence-activated droplet sorting; high-throughput screening; natural product
MeSH: Actinobacteria/metabolism*; High-Throughput Screening Assays/methods*; Genetic Engineering/methods*; Biological Products/metabolism*; Flow Cytometry; Metabolic Engineering/methods*
From: Chinese Journal of Biotechnology 2025;41(9):3375-3386
CountryChina
Language:Chinese
Abstract: Actinomycetes are important producers of high-value natural products, and the engineering of actinomycetes to enhance the biosynthesis of target natural products has long been a hot research topic in the scientific community. However, non-rational engineering methods suffer from low beneficial mutation rates, which limit the efficiency of mutant screening. The integration of high-throughput screening (HTS) technologies can effectively enhance the screening efficiency of elite mutants and significantly shorten the cycle of actinomycete strain engineering. This review comprehensively discusses various HTS technologies suitable for the engineering of actinomycete strains and compares them in terms of application scenarios, advantages, and disadvantages. HTS technologies include microplate-based screening, antimicrobial activity screening, antibiotic resistance screening, fluorescence-activated cell sorting (FACS), and fluorescence-activated droplet sorting (FADS). Additionally, this review summarizes the applications of these technologies in assisting actinomycete strain engineering and enhancing the yields of target compounds. The development and application of HTS technologies have not only facilitated the exploration of natural product resources in actinomycetes but also provided strong support for the rapid and efficient construction of high-performance engineered actinomycete strains.