Human umbilical cord mesenchymal stem cell-derived exosomes loaded with miR-132-3p promote skin wound healing.

Shuyue MENG; Xiaoning LI; Zhao YANG; Lei WANG

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Human umbilical cord mesenchymal stem cell-derived exosomes loaded with miR-132-3p promote skin wound healing.

Author: Shuyue MENG ¹ ; Xiaoning LI ¹ ; Zhao YANG ¹ ; Lei WANG ¹
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1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Publication Type:Journal Article
Keywords: exosomes; human umbilical cord mesenchymal stem cell; miR-132-3p; skin wound healing
MeSH: Humans; MicroRNAs/pharmacology*; Exosomes/metabolism*; Mesenchymal Stem Cells/cytology*; Wound Healing; Umbilical Cord/cytology*; Cell Proliferation; Fibroblasts/cytology*; Skin/injuries*; Cell Movement; Reactive Oxygen Species/metabolism*; Cells, Cultured
From: Chinese Journal of Biotechnology 2025;41(8):3110-3121
CountryChina
Language:Chinese
Abstract: Chronic non-healing wounds significantly impair patient rehabilitation and remain a critical clinical challenge. Stem cell-derived exosomes, owing to their biocompatibility and physiological activity, have emerged as a promising therapeutic approach in regenerative medicine. Beyond their intrinsic wound-healing properties, exosomes are increasingly explored as carriers for small-molecule drugs to enhance synergistic treatment effects. Although microRNAs (miRNAs) exhibit potential in promoting cell proliferation and re-epithelialization, their clinical application is hindered by poor stability. In this study, we investigated the therapeutic effects of miR-132-3p-loaded human umbilical mesenchymal stem cell-derived exosomes (miR-132-3p@UMSC-EXOs) on human foreskin fibroblast-1 (HFF-1). Our findings demonstrated that miR-132-3p@UMSC-EXOs significantly enhanced proliferation and migration of HFF-1, while reducing intracellular reactive oxygen species (ROS) levels compared with unloaded exosomes. Furthermore, qRT-PCR and Western blotting analyses revealed that miR-132-3p@UMSC-EXOs modulated the expression of genes associated with extracellular matrix (ECM) remodeling and inflammation, suggesting their potential to upregulate collagen synthesis and improve ECM metabolism. These results highlight the therapeutic promise of miR-132-3p@UMSC-EXOs in accelerating wound healing.