Advances in phage immunoprecipitation sequencing technology.
- Author:
Yuhao ZHU
1
;
Wenlong ZHU
1
;
Yujie LAI
1
;
Mengjia ZHANG
1
;
Wentao LI
1
Author Information
- Publication Type:English Abstract
- Keywords: diagnostic techniques; peptide library; phage immunoprecipitation sequencing (PhIP-Seq); vaccine development
- MeSH: Peptide Library; Humans; Immunoprecipitation/methods*; High-Throughput Nucleotide Sequencing/methods*; Bacteriophages/genetics*
- From: Chinese Journal of Biotechnology 2025;41(8):2987-3007
- CountryChina
- Language:Chinese
- Abstract: Phage immunoprecipitation sequencing (PhIP-Seq) is a high-throughput and low-cost method for analyzing the specific binding of target proteins to peptide libraries. The method uses oligonucleotide library synthesis (OLS) to encode proteome-scale peptide libraries for display on phages, and then immunoprecipitates these library phages with target proteins (such as antibodies) for subsequent analysis by high-throughput DNA sequencing. PhIP-Seq enables the screening of peptide targets that react specifically with hundreds of proteins or pathogens. PhIP-Seq has been successfully applied in various fields such as disease detection, screening of autoimmune disease biomarkers, vaccine development, and allergen detection, becoming a high-throughput diagnostic technology. This article systematically describes the development, applications, and result evaluation of PhIP-Seq, in order to gain a more comprehensive understanding of the application and future development prospects of this technology in various fields.
