Identification of PLATZ gene family in <i>Camellia sinensis</i> and expression analysis of this gene family under high temperature and drought stresses.

Xiaoshu YI; Anru ZHENG; Chengzhe ZHOU; Caiyun TIAN; Cheng ZHANG; Yuqiong GUO; Xuan CHEN

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Identification of PLATZ gene family in Camellia sinensis and expression analysis of this gene family under high temperature and drought stresses.

Author: Xiaoshu YI ¹ ; Anru ZHENG ² ; Chengzhe ZHOU ² ; Caiyun TIAN ² ; Cheng ZHANG ² ; Yuqiong GUO ² ; Xuan CHEN ¹
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1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
2. Anxi College of Tea Science/College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
Publication Type:Journal Article
Keywords: Camellia sinensis; PLATZ gene family; bioinformatics analysis; drought; high temperature
MeSH: Droughts; Camellia sinensis/physiology*; Phylogeny; Gene Expression Regulation, Plant; Plant Proteins/genetics*; Stress, Physiological/genetics*; Multigene Family; Transcription Factors/genetics*; Hot Temperature; Genes, Plant
From: Chinese Journal of Biotechnology 2025;41(7):2897-2912
CountryChina
Language:Chinese
Abstract: The plant AT-rich sequence and zinc-binding protein (PLATZ) family is composed of plant-specific zinc finger-like transcription factors, which play important roles in plant growth, development, and stress tolerance. In this study, to gain a better understanding of the PLATZ gene in C. sinensis and elucidate its response under drought and high temperature conditions, the PLATZ gene family of the C. sinensis cultivar 'Tieguanyin' was systematically identified, and a total of 12 CsPLATZ family members were identified. Expasy online and other bioinformatics tools were used to analyze the members of the PLATZ gene family in terms of protein physicochemical properties, phylogenetic relationships, cis-acting elements, gene structures, and intra- and inter-species collinearity. The results of phylogenetic analysis classified the CsPLATZ family members into 2 subfamilies. The conserved domains and gene structures of PLATZ family members within the same subfamily had a high degree of consistency, whereas a certain degree of diversity was observed among the subfamilies. Twelve PLATZ genes were unevenly distributed across 7 chromosomes of C. sinensis and the promoter regions of these genes had multiple cis-acting elements related to hormone and stress responses. The collinearity analysis showed that there were 4 pairs of duplication events in the CsPLATZ gene family, all of which were segmental duplications. Based on this gene family, C. sinensis had a closer evolutionary relationship with A. thaliana than with O. sativa. The transcriptome analysis showed that the expression levels of CsPLATZ family members varied in different tissue samples of C. sinensis. 6 genes (CsPLATZ-1, CsPLATZ-2, CsPLATZ-3, CsPLATZ-4, CsPLATZ-6, and CsPLATZ-8) with high expression in shoots, young leaves, and roots were selected for high temperature and drought stress treatments, and their expression was quantified by qRT-PCR. The results indicated that the six genes might play important roles in the response to drought stress. In addition, CsPLATZ-2 and CsPLATZ-8 might have important functions in the response to high temperature stress. The results of this study will contribute to a better understanding of the biological functions of PLATZ genes and their possible roles in the growth, development, and stress responses of C. sinensis.