Expression pattern of polyhedrin of BmNPV and involvement of importin α in the nuclear import of Polh.
- Author:
Jiale LI
1
;
Xingyang WANG
1
;
Xiaofeng WU
1
Author Information
- Publication Type:Journal Article
- Keywords: Bombyx mori nucleopolyhedrovirus; importin α; nuclear import; polyhedrin
- MeSH: Nucleopolyhedroviruses/metabolism*; Active Transport, Cell Nucleus; Animals; Bombyx/virology*; alpha Karyopherins/metabolism*; Cell Nucleus/metabolism*; Viral Structural Proteins/metabolism*; Occlusion Body Matrix Proteins
- From: Chinese Journal of Biotechnology 2025;41(7):2647-2657
- CountryChina
- Language:Chinese
- Abstract: Bombyx mori nucleopolyhedrovirus (BmNPV) is extremely harmful to the silk industry. The polyhedrin, which encodes the polyhedrin (Polh), can be expressed at ultra-high levels and form occlusion bodies in the nucleus, embedding the progeny virus within it. However, the detailed mechanism by which polyhedrin is transported into the host cell nucleus remains unknown. Clarifying the nuclear import mechanisms of viral proteins can help us develop better prevention and treatment measures against baculoviruses. This study employed molecular cloning, co-immunoprecipitation, and immunofluorescence to analyze in detail the expression pattern of the highly expressed polyhedrin in the very late stage of the virus, and further revealed that the host protein importin α participates in the nuclear import of polyhedrin through protein interactions. This study provides a reference for further elucidating the nuclear import mechanisms of the baculovirus proteins including polyhedrin that can enter the nucleus.
