Expression efficiency of three DNA plamids and their mRNAs expressing foot-and-mouth disease virus (FMDV) antigenic proteins.

Lixin JIANG; Haiyun LIU; Yifan LIU; Yuqing MA; Shiqi SUN; Zezhong ZHENG; Huichen GUO

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Expression efficiency of three DNA plamids and their mRNAs expressing foot-and-mouth disease virus (FMDV) antigenic proteins.

Author: Lixin JIANG ¹ ; Haiyun LIU ² ; Yifan LIU ² ; Yuqing MA ² ; Shiqi SUN ² ; Zezhong ZHENG ¹ ; Huichen GUO ²
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1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, China.
2. State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China.
Publication Type:Journal Article
Keywords: expression of protein; foot-and-mouth disease virus (FMDV); non-replicating mRNA; untranslated region
MeSH: Foot-and-Mouth Disease Virus/genetics*; Animals; RNA, Messenger/biosynthesis*; Foot-and-Mouth Disease/immunology*; Antigens, Viral/biosynthesis*; Viral Vaccines/biosynthesis*; Genetic Vectors/genetics*; Cell Line; Vaccines, DNA/immunology*
From: Chinese Journal of Biotechnology 2025;41(7):2623-2633
CountryChina
Language:Chinese
Abstract: Foot-and-mouth disease (FMD) is one of the major animal infectious diseases in the world. All cloven-hoofed animals are susceptible to FMD. Vaccination is still the first choice for the prevention and control of FMD. mRNA vaccines can be rapidly designed, synthesized, and produced on a large scale in vitro, and they can induce effective protective immune responses, demonstrating the advantages of rapid development, easy preparation, and low biosafety risks. The design of untranslated regions is a key to enhancing the expression and efficacy of mRNA vaccines. In order to generate an efficient FMD mRNA vaccine, we designed three FMD P12A3C expression vectors with different untranslated regions and synthesized corresponding mRNAs. By comparing expression efficiency of these vectors and their mRNAs at different time points and in different cell lines, we found that the mRNA P12A3C-UTR3 had the best expression and universality. This study laid a foundation for the development of mRNA vaccines against FMD and provided a theoretical basis for the optimal sequence design of efficient mRNA.