Construction of an intein-mediated Split-Cre system.

Yifei AO; Qi ZHANG; Yuxi CHEN; Junjiu HUANG; Jinkun WEN

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Construction of an intein-mediated Split-Cre system.

Author: Yifei AO ¹ ; Qi ZHANG ² ; Yuxi CHEN ³ ; Junjiu HUANG ³ ; Jinkun WEN ²
Author Information

1. School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China.
2. School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China.
3. School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, Guangdong, China.
Publication Type:Journal Article
Keywords: Cre; Rhodothermus marinus (Rma) intein; Split-Cre; Split-intein
MeSH: Inteins/genetics*; Animals; Integrases/biosynthesis*; Mice; Dependovirus/metabolism*; Bacterial Proteins/genetics*; Recombination, Genetic; Humans
From: Chinese Journal of Biotechnology 2025;41(4):1490-1499
CountryChina
Language:Chinese
Abstract: The Split-Cre system consists of two inactive polypeptides: NCre and CCre, which can be recombined into an active full-length Cre under certain conditions. This system is typically used with LoxP. To develop an efficient Split-Cre system, this study used Rma intein from Rhodothermus marinus to split Cre and screened out the split site S102 which could efficiently mediate the recombination of Cre in the "Traffic Light" reporter cell line. Moreover, the S102 Split-Cre system was delivered to mice by dual-adeno-associated virus (AAV), and it was demonstrated that the efficiency of the Rma intein-mediated S102 Split-Cre system was comparable to the full-length Cre in mice. This system lays a foundation for both basic and applied research on Split-Cre.