Preparation, optimization, and <i>in vitro</i> evaluation of <i>Pediococcus acidilactici</i> HRQ-1 microcapsules.

Ruiqin HAN; Song XU; Xinyuan WANG; Jingjing WANG; Xiaoxia ZHANG; Liping DU; Zhiyong HUANG

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Preparation, optimization, and in vitro evaluation of Pediococcus acidilactici HRQ-1 microcapsules.

Author: Ruiqin HAN ¹ ; Song XU ² ; Xinyuan WANG ² ; Jingjing WANG ² ; Xiaoxia ZHANG ² ; Liping DU ¹ ; Zhiyong HUANG ²
Author Information

1. School of Biological Engineering, Tianjin University of Science and Technology, Tianjin 300000, China.
2. Tianjin Key Laboratory for Industrial Biosystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300000, China.
Publication Type:Journal Article
Keywords: Pediococcus acidilactici; formula and process; in vitro evaluation; live bacterial drugs; microcapsules
MeSH: Pediococcus acidilactici/chemistry*; Probiotics/chemistry*; Capsules/chemistry*; Alginates/chemistry*; Chitosan/chemistry*; Drug Compounding/methods*; Glucuronic Acid/chemistry*; Hexuronic Acids/chemistry*; Freeze Drying
From: Chinese Journal of Biotechnology 2025;41(4):1415-1427
CountryChina
Language:Chinese
Abstract: We have isolated an intestinal probiotic strain, Pediococcus acidilactici HRQ-1. To improve its gastrointestinal fluid tolerance, transportation and storage stability, and slow-release properties, we employed the extrusion method to prepare the microcapsules with P. acidilactici HRQ-1 as the core material and sodium alginate and chitosan as the wall material. The optimal conditions for preparing the microcapsules were determined by single factor and orthogonal tests, and the optimal ratio was determined by taking the embedding rate, survival rate, storage stability, gastrointestinal fluid tolerance, and release rate as the evaluation indexes. The results showed that under the optimal embedding conditions, the embedding rate reached (89.60±0.02)%. Under the optimal formula of freeze-drying protective agent, the freeze-drying survival rate reached (76.42±0.13)%, and the average size of the microcapsules produced was (1.16±0.03) mm. The continuous gastrointestinal fluid simulation experiments confirmed that the microcapsules ensured the viable bacterial count and can slowly release bacteria in the intestinal fluid. The curve of the viable bacterial count during storage at 4 ℃ and room temperature indicated that the prepared microcapsules achieved strains' live number protection. The formula and preparation process of P. acidilactici microcapsules may provide a technological reserve for the preparation of more live bacterial drugs in the future.