LBD gene family in <i>Hippophae rhamnoides</i>: identification and expression pattern during flower bud development.

Xinjuan LI; Panpan YANG; Tian ZHANG; Qiandan REN; Wu ZHOU

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LBD gene family in Hippophae rhamnoides: identification and expression pattern during flower bud development.

Author: Xinjuan LI ¹ ; Panpan YANG ² ; Tian ZHANG ¹ ; Qiandan REN ¹ ; Wu ZHOU ¹
Author Information

1. College of Eco-environmental Engineering, Qinghai University, Xining 810016, Qinghai, China.
2. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China.
Publication Type:Journal Article
Keywords: Hippophae rhamnoides; flower bud development; gene expression; lateral organ boundaries (LOB) domain (LBD) gene family; reverse transcription-polymerase chain reaction
MeSH: Flowers/genetics*; Hippophae/metabolism*; Phylogeny; Gene Expression Regulation, Plant; Plant Proteins/genetics*; Transcription Factors/genetics*; Multigene Family; Genes, Plant
From: Chinese Journal of Biotechnology 2025;41(2):753-770
CountryChina
Language:Chinese
Abstract: Lateral organ boundaries (LOB) domain (LBD) genes encode a family of transcription factors ubiquitous in higher plants, playing crucial roles in the growth, development, and stress responses. Hippophae rhamnoides, known for its drought, cold, and saline-alkali tolerance, offers significant economic benefits and ecological values. Utilizing the whole genome data and bioinformatics approaches, this study identified and analyzed the LBD gene family in H. rhamnoides. Additionally, we examined the expression pattern of HrLBD genes by integrating the transcriptome data from male and female flower buds in development. Eleven LBD genes were identified in H. rhamnoides, and these genes were distributed on five chromosomes. The HrLBD proteins showed the lengths ranging from 159 aa to 302 aa, the molecular weights between 18 249.91 Da and 33 202.01 Da, and the subcellular localization in the nucleus or chloroplasts. LBD protein domains and gene structures were highly conserved, featuring similar motifs. The phylogenetic analysis of HrLBD genes and the LBD genes in Arabidopsis thaliana and Hordeum vulgare revealed that HrLBD genes falled into two major categories: Class Ⅰ and Class Ⅱ. The transcriptome data and RT-qPCR showed that HrLBD genes were highly expressed in male flower buds, with up-regulated expression levels throughout bud development, indicating a role in the specific stage of male flower bud development. This study lays a theoretical foundation for exploring the roles of HrLBD genes in the growth, development, and sex differentiation of H. rhamnoides flower buds.