Genome-wide identification, characterization, and expression analysis of MAPK genes in response to <i>Plasmodiophora brassicae</i> infection in <i>Brassica juncea</i>.

Chu XU; Haiping WANG; Jiangping SONG; Xiaohui ZHANG; Huixia JIA; Jiaqi HAN; Zhijie LI; Sen LI; Wenlong YANG

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Genome-wide identification, characterization, and expression analysis of MAPK genes in response to Plasmodiophora brassicae infection in Brassica juncea.

Author: Chu XU ¹ ; Haiping WANG ² ; Jiangping SONG ² ; Xiaohui ZHANG ² ; Huixia JIA ² ; Jiaqi HAN ¹ ; Zhijie LI ² ; Sen LI ¹ ; Wenlong YANG ²
Author Information

1. College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China.
2. State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Publication Type:Journal Article
Keywords: Brassica juncea; clubroot disease; expression analysis; mitogen-activated protein kinases
MeSH: Mustard Plant/parasitology*; Plasmodiophorida/pathogenicity*; Plant Diseases/genetics*; Mitogen-Activated Protein Kinases/metabolism*; Phylogeny; Disease Resistance/genetics*; Gene Expression Regulation, Plant; Genome, Plant; Plant Proteins/genetics*
From: Chinese Journal of Biotechnology 2025;41(2):736-752
CountryChina
Language:Chinese
Abstract: In recent years, the spread of clubroot disease caused by Plasmodiophora brassicae infection has seriously affected the yield and quality of Brassica juncea (L.) Czern.. The cascade of mitogen-activated protein kinases (MAPKs), a highly conserved signaling pathway, plays an important role in plant responses to both biotic and abiotic stress conditions. To mine the MAPK genes related to clubroot disease resistance in B. juncea, we conducted a genome-wide analysis on this vegetable, and we analyzed the phylogenetic evolution and gene structure of the MAPK gene family in mustard. The 66 BjuMAPK genes identified by screening the whole genome sequence of B. juncea were unevenly distributed on 17 chromosomes. At the genomic scale, tandem repeats led to an increase in the number of MAPK genes in B. juncea. It was found that members of the same subfamily had similar gene structures, and there were great differences among different subfamilies. These predicted cis-acting elements were related to plant hormones, stress resistance, and plant growth and development. The expression of BjuMAPK02, BjuMAPK15, BjuMAPK17, and BjuMAPK19 were down-regulated or up-regulated in response to P. brassicae infection. The above results lay a theoretical foundation for further studying the functions of BjuMAPK genes in B. juncea in response to the biotic stress caused by clubroot disease.