Selenium nanoparticles synthesized by <i>Streptomyces avermitilis</i>: physical and chemical characteristics and inhibitory activity on a pathogen of <i>Lycium barbarum</i>.

Qi ZHANG; Yani LI; Rongjuan ZHOU; Jiayuan QING; Sijun YUE

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Selenium nanoparticles synthesized by Streptomyces avermitilis: physical and chemical characteristics and inhibitory activity on a pathogen of Lycium barbarum.

Author: Qi ZHANG ¹ ; Yani LI ¹ ; Rongjuan ZHOU ¹ ; Jiayuan QING ¹ ; Sijun YUE ²
Author Information

1. Xinhua College of Ningxia University, Yinchuan 750021, Ningxia, China.
2. Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, School of Life Sciences, Ningxia University, Yinchuan 750021, Ningxia, China.
Publication Type:Journal Article
Keywords: Fusarium oxysporum; Streptomyces avermitilis; inhibitory activity; physical and chemical characterization; selenium nanoparticles
MeSH: Streptomyces/metabolism*; Fusarium/drug effects*; Lycium/microbiology*; Selenium/metabolism*; Nanoparticles/chemistry*; Plant Diseases/microbiology*; Metal Nanoparticles/chemistry*; Antifungal Agents/pharmacology*
From: Chinese Journal of Biotechnology 2025;41(2):693-705
CountryChina
Language:Chinese
Abstract: Biosynthesized selenium nanoparticles (SeNPs) have attracted much attention because of their unique physical, chemical, and biological properties. The microbial reduction of selenium salts to SeNPs has great potential, while there is a lack of elite strains. In this study, we explored the reduction of Na₂SeO₃ by Streptomyces avermitilis into SeNPs. The colonies and hyphae of the strain and the synthesized SeNPs were characterized by optical microscopy, scanning electron microscopy (SEM), transmission electron microscope (TEM), energy dispersive spectrometry (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). At the same time, the inhibitory activity of SeNPs on Fusarium oxysporum, the main pathogen causing root rot of Lycium barbarum, was studied. The results showed that S. avermitilis converted Na₂SeO₃ into SeNPs and tolerated 300 mmol/L Na₂SeO₃, demonstrating strong tolerance. S. avermitilis synthesized spherical SeNPs in the cytoplasm, and most of SeNPs had a diameter of about 100 nm and were released by hyphal fracture. The SeNPs synthesized by S. avermitilis were amorphous, and their surfaces were dominated by C and Se, with the existence of O, N and other elements. SeNPs had functional groups such as -OH, C=O, C-N, and C-H, which were closely related to the stability and biological activity of SeNPs. The SeNPs synthesized by S. avermitilis showcased significant inhibitory activity on F. oxysporum, and 25.0 μmol/mL SeNPs showcased the inhibition rate of 77.61% and EC₅₀ of 0.556 μmol/mL. In conclusion, S. avermitilis can tolerate high Na₂SeO₃ stress and mediate the synthesis of SeNPs. The synthesized SeNPs have good stability and strong inhibitory activity, demonstrating the potential application value in the preparation of SeNPs and the control of L. barbarum root rot.