Partial knockout of NtPDK1a/1b/1c/1d enhances the disease resistance of Nicotiana tabacum.

Qianwei REN; Hujiao LAN; Tianyao LIU; Huanting ZHAO; Yating ZHAO; Rui ZHANG; Jianzhong LIU

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Partial knockout of NtPDK1a/1b/1c/1d enhances the disease resistance of Nicotiana tabacum.

Author: Qianwei REN ¹ ; Hujiao LAN ¹ ; Tianyao LIU ¹ ; Huanting ZHAO ¹ ; Yating ZHAO ¹ ; Rui ZHANG ¹ ; Jianzhong LIU ¹
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1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Publication Type:Journal Article
Keywords: AGC kinase; CRISPR/Cas9 technology; NtPDK1; plant immunity; tobacco
MeSH: Nicotiana/virology*; Disease Resistance/genetics*; Plant Diseases/immunology*; Plants, Genetically Modified/genetics*; Gene Knockout Techniques; Plant Proteins/genetics*; CRISPR-Cas Systems; Protein Serine-Threonine Kinases/genetics*; 3-Phosphoinositide-Dependent Protein Kinases/genetics*; Pyruvate Dehydrogenase Acetyl-Transferring Kinase; Tobacco Mosaic Virus/pathogenicity*
From: Chinese Journal of Biotechnology 2025;41(2):670-679
CountryChina
Language:Chinese
Abstract: The protein kinase A/protein kinase G/protein kinase C-family (AGC kinase family) of eukaryotes is involved in regulating numerous biological processes. The 3-phosphoinositide- dependent protein kinase 1 (PDK1), is a conserved serine/threonine kinase in eukaryotes. To understand the roles of PDK1 homologous genes in cell death and immunity in tetraploid Nicotiana tabacum, the previuosly generated transgenic CRISPR/Cas9 lines, in which 5-7 alleles of the 4 homologous PDK1 genes (NtPDK1a/1b/1c/1d homologs) simultaneously knocked out, were used in this study. Our results showed that the hypersensitive response (HR) triggered by transient overexpression of active Pto (Pto^Y207D) or soybean GmMEKK1 was significantly delayed, whereas the resistance to Pseudomonas syrangae pv. tomato DC3000 (Pst DC3000) and tobacco mosaic virus (TMV) was significantly elevated in these partial knockout lines. The elevated resistance to Pst DC3000 and TMV was correlated with the elevated activation of NtMPK6, NtMPK3, and NtMPK4. Taken together, our results indicated that NtPDK1s play a positive role in cell death but a positive role in disease resistance, likely through negative regulation of the MAPK signaling cascade.