Research progress in the engineering strains for producing double-stranded RNA.

Author: Jincheng CUI ¹ ; Jie CUI ¹ ; Xiaoying BIAN ¹
Author Information

1. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, Shandong, China.
Publication Type:English Abstract
Keywords: RNA interference; chassis; dsRNA; engineering strain
MeSH: RNA, Double-Stranded/genetics*; Genetic Engineering/methods*; RNA Interference; Pesticides; Animals
From: Chinese Journal of Biotechnology 2025;41(2):546-558
CountryChina
Language:Chinese
Abstract: Ensuring food security requires new green pesticides. Double-stranded RNA (dsRNA) pesticides trigger RNA interference by exogenous dsRNA specifically targeting pests and diseases. They can inhibit the expression of key genes in pathogens or pests, thereby achieving effective control of specific pests and diseases. DsRNA pesticides are environmentally friendly, with strong specificity and efficient gene silencing ability, while they have problems such as high production costs. Using engineering strains to produce dsRNA is a feasible strategy, whereas currently there is no cost-effective engineering strain for producing dsRNA. This article reviews the research progress and production strategies of using microorganisms to produce dsRNA, hoping to provide reference for dsRNA production.