Efficient synthesis of polydatin by a two-enzyme coupled with one-pot method.

Jingli DAI; Zixu YAN; Kexue ZHAO; Xiaoli LI; Yongjun ZANG; Qilin XU; Fucheng ZHU

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Efficient synthesis of polydatin by a two-enzyme coupled with one-pot method.

Author: Jingli DAI ¹ ; Zixu YAN ¹ ; Kexue ZHAO ¹ ; Xiaoli LI ¹ ; Yongjun ZANG ² ; Qilin XU ² ; Fucheng ZHU ²
Author Information

1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, Anhui, China.
2. School of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, Anhui, China.
Publication Type:Journal Article
Keywords: fed-batch strategy; glycosyltransferase; one-pot coupling reaction; polydatin; uridine diphosphate-glucose regeneration
MeSH: Stilbenes/chemistry*; Glucosides/biosynthesis*; Resveratrol; Fallopia japonica/chemistry*; Glycosyltransferases/genetics*
From: Chinese Journal of Biotechnology 2025;41(1):461-473
CountryChina
Language:Chinese
Abstract: Traditional Chinese medicine of Polygonum cuspidatum has been utilized in China for thousands of years. Its primary active compound, polydatin, exhibits a variety of pharmacological effects including the regulation of glucose and lipid metabolism, suppression of cough and asthma, as well as antibacterial and anti-inflammatory properties. However, conventional methods for polydatin production are inadequate to satisfy the market demand. This study aims to explore the green and efficient preparation of polydatin. With resveratrol as the substrate, we efficiently synthesized polydatin by using the triple mutant IGW (Y14I/I62G/M315W) of the glycosyltransferase UGT_BS based on a strategy of two-enzyme coupled with one-pot and realized the recycling of uridine diphosphate-glucose (UDPG). The conditions of the two-enzyme reaction were optimized. Under the conditions of 35 ℃, pH 8.0, IGW: AtSuSy1 activity ratio of 3:4, dimethyl sulfoxide (DMSO) volume fraction of 5%, uridine diphosphate (UDP) concentration of 0.10 mmol/L, and sucrose concentration of 0.6 mol/L, the conversion of 2 mmol/L resveratrol reached 80.6% within 1 h, and the proportion of polydatin was over 90%. This study achieved the recycling of UDPG via a two-enzyme coupling system and shortened the reaction time. At the same time, the fed-batch strategy was adopted, and the yield of polydatin reached 6.28 g/L after 24 h in the one-pot coupling reaction, which provided a new strategy for green and efficient preparation of polydatin.