Construction and application of an inducible transcriptional regulatory tool from <i>Medicago truncatula</i> in <i>Saccharomyces cerevisiae</i>.

Meilin FENG; Caifang SHI; Ying WANG; Chun LI

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Construction and application of an inducible transcriptional regulatory tool from Medicago truncatula in Saccharomyces cerevisiae.

Author: Meilin FENG ¹ ; Caifang SHI ¹ ; Ying WANG ¹ ; Chun LI ¹
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1. Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Publication Type:Journal Article
Keywords: Saccharomyces cerevisiae; regulatory elements; synthetic biology; transcription factors; β-carotene
MeSH: Medicago truncatula/metabolism*; Saccharomyces cerevisiae/metabolism*; Transcription Factors/genetics*; beta Carotene/biosynthesis*; Promoter Regions, Genetic/genetics*; Gene Expression Regulation, Plant; Metabolic Engineering/methods*; Regulatory Elements, Transcriptional/genetics*; Plant Proteins/genetics*
From: Chinese Journal of Biotechnology 2025;41(1):363-375
CountryChina
Language:Chinese
Abstract: Transcriptional regulation based on transcription factors is an effective regulatory method widely used in microbial cell factories. Currently, few naturally transcriptional regulatory elements have been discovered from Saccharomyces cerevisiae and applied. Moreover, the discovered elements cannot meet the demand for specific metabolic regulation of exogenous compounds due to the high background expression or narrow dynamic ranges. There are abundant transcriptional regulatory elements in plants. However, the sequences and functions of most elements have not been fully characterized and optimized. Particularly, the applications of these elements in microbial cell factories are still in the infancy stage. In this study, natural regulatory elements from Medicago truncatula were selected, including the transcription factors MtTASR2 and MtTASR3, along with their associated promoter P_roHMGR1, for functional characterization and engineering modification. We constructed an inducible transcriptional regulation tool and applied it in the regulation of heterologous β-carotene synthesis in S. cerevisiae, which increased the β-carotene production by 7.31 folds compared with the original strain. This study demonstrates that plant-derived transcriptional regulatory elements can be used to regulate the expression of multiple genes in S. cerevisiae, providing new strategies and ideas for the specific regulation and application of these elements in microbial cell factories.