Promotion of <i>Stenotrophomonas</i> sp. on the photosynthetic growth of microalgae exposed to high concentrations of formate.

Mengmeng XING; Weijie ZHENG; Wangyin WANG; Xupeng CAO; Can LI

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Promotion of Stenotrophomonas sp. on the photosynthetic growth of microalgae exposed to high concentrations of formate.

Author: Mengmeng XING ¹ ; Weijie ZHENG ¹ ; Wangyin WANG ¹ ; Xupeng CAO ¹ ; Can LI ¹
Author Information

1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
Publication Type:Journal Article
Keywords: bacterium-microalga interaction; formate; protein; security of food supply; solar fuels
MeSH: Photosynthesis/physiology*; Formates/pharmacology*; Stenotrophomonas/growth & development*; Microalgae/metabolism*; Chlamydomonas reinhardtii/growth & development*
From: Chinese Journal of Biotechnology 2025;41(1):230-241
CountryChina
Language:Chinese
Abstract: Formate is an important solar fuel, with large application potential in bioconversion. Especially, the win-win collaboration is achieved when formate is applied to the cultivation of microalgae, which combines the advantages from both artificial and natural photosynthesis. However, the inhibition of formate on the photosynthetic electron transport hinders the application of formate at high concentrations. The engineering or directed evolution of the regulation pathway is a case-by-case and time-consuming strategy. Here, we developed a new strategy by introducing a Stenotrophomonas sp. strain which was isolated and identified from the long-term self-evolution process of Chlamydomonas reinhardtii for adapting to high concentrations of formate. The co-culture with the strain or the fermentation broth relieved the inhibition of formate (50 mmol/L) on C. reinhardtii and promoted the growth of the microalga. Especially, the protein content increased significantly to nearly 50% of the dried weight. In addition, the co-culture also benefited the growth of both Chlorella pyrenoidesa and Synechocystis sp. PCC 6803 exposed to formate, which indicated broader applicability of this strategy. This strategy provides the opportunity to overcome the bottleneck in the formate-mediated artificial-natural hybrid photosynthesis and to aid the development of technologies for solar energy-driven production of bulk biomass, including proteins, by carbon dioxide reduction.