Research progress and application of nanopores in single-molecule protein sensing.

Lingzhi WU; Xingyue LUO; Haofeng DENG; Qianli MA; Lihua TANG

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Research progress and application of nanopores in single-molecule protein sensing.

Author: Lingzhi WU ¹ ; Xingyue LUO ¹ ; Haofeng DENG ² ; Qianli MA ² ; Lihua TANG ³
Author Information

1. College of Science, Nanjing University of Posts and Telecommunications, Nanjing 210023, Jiangsu, China.
2. College of Integrated Circuit Science and Engineering, Nanjing University of Posts and Telecommunications, Nanjing 210023, Jiangsu, China.
3. School of Computer Science, Nanjing University of Posts and Telecommunications, Nanjing 210023, Jiangsu, China.
Publication Type:English Abstract
Keywords: nanopore; protein conformation; protein folding; protein interaction; single-molecule level
MeSH: Nanopores; Proteins/chemistry*; Biosensing Techniques/methods*; Protein Folding; Humans
From: Chinese Journal of Biotechnology 2025;41(1):92-116
CountryChina
Language:Chinese
Abstract: Proteins are fundamental carriers as the structural elements and biochemically active entities responsible for catalysis, transport, and regulation. These functions are depending on the protein folding into precise three-dimensional structures, interacting with ligands, and conformational changes. This article reviews the recent progress of nanopores in single-molecule protein sensing, involving the identification of polypeptides and proteins, the conformation changes of protein folding, the molecular structure responsible to the pH of solutions, the molecular interactions, and protein sequencing. These studies provide clues to understand life activities and facilitate the early diagnosis of diseases and design of drugs for precise treatment.