Construction of a muscle-specific synthetic promoter library and correlation analysis of the element composition and activity of highly active promoters.
- Author:
Zening WANG
1
;
Mingfeng JIANG
1
;
Jiu QU
2
;
Xiaowei LI
3
;
Yili LIU
1
Author Information
- Collective Name:Daojieriqing;Danbaciren
- Publication Type:Journal Article
- Keywords: correlation analysis; promoter elements; screening; synthetic promoter
- MeSH: Promoter Regions, Genetic; Humans; Animals; Mice; Gene Library; Cell Line; Transfection; HeLa Cells; Luciferases/metabolism*; Muscle, Skeletal/metabolism*; Plasmids/genetics*; Myoblasts/cytology*
- From: Chinese Journal of Biotechnology 2024;40(12):4616-4627
- CountryChina
- Language:Chinese
- Abstract: The purpose of this study is to construct a muscle-specific synthetic promoter library, screen out muscle-specific promoters with high activity, analyze the relationship between element composition and activity of highly active promoters, and provide a theoretical basis for artificial synthesis of promoters. In this study, 19 promoter fragments derived from muscle-specific elements, conserved elements, and viral regulatory sequences were selected and randomLy connected to construct a muscle-specific synthetic promoter library. The luciferase plasmids pCMV-Luc and pSPs-Luc were constructed and transfected into the myoblast cell line C2C12. The activities of the synthesized promoters were evaluated by the luciferase activity assay. Two non-muscle-derived cell lines HeLa and 3T3 were used to verify the muscle specificity of the highly active promoters. The sequences of promoters with high activity, good muscle specificity, and correct sequences were analyzed to explore the relationship between the element composition and activity of promoters. We successfully constructed a muscle-specific promoter library and screened out 321 effective synthetic promoter plasmids. Among them, the activity of SP-301 promoter was 5.63 times that of CMV. The 15 promoters with high activity were muscle-specific. In the promoters with high activity and correct sequences, there was a relationship between their element composition and activity. Muscle-specific elements accounted for a high proportion in the promoters, while they had weak correlations with the promoter activity, being tissue-specific determinants. Viral elements accounted for no less than 20% in highly active promoters, which may be the key elements for the promoter activity. The content of conserved elements was proportional to the promoter activity. This study lays a theoretical foundation for the synthesis of tissue-specific efficient promoters and provides a new idea for the construction and application of in-situ gene delivery systems.
