Screening and identification of host proteins interacting with the non-structural protein 15 (Nsp15) of porcine epidemic diarrhea virus.

Jinlei SUN; Ruiming YU; Liping ZHANG; Zhongwang ZHANG; Yonglu WANG; Li PAN; Quanwei ZHANG; Xinsheng LIU

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Screening and identification of host proteins interacting with the non-structural protein 15 (Nsp15) of porcine epidemic diarrhea virus.

Author: Jinlei SUN ¹ ; Ruiming YU ² ; Liping ZHANG ² ; Zhongwang ZHANG ² ; Yonglu WANG ² ; Li PAN ² ; Quanwei ZHANG ¹ ; Xinsheng LIU ²
Author Information

1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
2. National Key Laboratory of Animal Disease Prevention and Control, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China.
Publication Type:Journal Article
Keywords: non-structural proteins; porcine epidemic diarrhea; protein-protein interaction; virus replication
MeSH: Porcine epidemic diarrhea virus/genetics*; Viral Nonstructural Proteins/metabolism*; Animals; Swine; Virus Replication; Coronavirus Infections/veterinary*; Swine Diseases/metabolism*
From: Chinese Journal of Biotechnology 2024;40(12):4533-4545
CountryChina
Language:Chinese
Abstract: To screen and identify the key host proteins interacting with the non-structural protein 15 (Nsp15) of porcine epidemic diarrhea virus (PEDV). The IP/pull-down assay and mass spectrometry were employed to screen and identify the host proteins interacting with Nsp15. The interaction between the host protein and Nsp15 was studied by co-immunoprecipitation and laser scanning confocal microscopy. Finally, Western blotting and RT-qPCR were employed to examine the interaction between SLC25a3 and PEDV. The recombinant eukaryotic expression vector pcDNA3.1(+)-Flag-Nsp15 was successfully constructed, and the host protein SLC25a3 interacting with PEDV Nsp15 was screened out. An interaction existed between SLC25a3 and Nsp15, and SLC25a3 significantly inhibited PEDV replication in a dose-dependent manner. SLC25a3 inhibits PEDV replication. The results of this study provide a basis for deciphering the role and mechanism of SLC25a3 in the host immune response to PEDV infection.